BD PharmingenTM Purified Mouse Anti-Human CD133

Description

Flow cytometric analysis of CD133 expression on human retinoblastoma cell line. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dotted line histogram or Purified Mouse Anti-Human CD133 antibody Cat. No. 567210; solid line histogram at 0.125 mug/test. The cells were washed and stained with PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Immunohistochemical analysis of human CD133 expression by cells comprising human placenta tissue. Cold acetone-fixed frozen sections of human placenta were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse Anti-Human CD133 antibody Cat. No. 567210; Right Panel at 5 mug/ml for one hour at room temperature. A three-step staining procedure that employs a Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 , Streptavidin-Horseradish Peroxidase Sav-HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The tissues were counterstained with hematoxylin. CD133 positive staining on syncytiotrophoblasts of chorionic villi Right Panel is indicated by the intense brown labeling. Original magnification: 20X. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD133 expression on human retinoblastoma cell line. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dotted line histogram or Purified Mouse Anti-Human CD133 antibody Cat. No. 567210; solid line histogram at 0.125 mug/test. The cells were washed and stained with PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Immunohistochemical analysis of human CD133 expression by cells comprising human placenta tissue. Cold acetone-fixed frozen sections of human placenta were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse Anti-Human CD133 antibody Cat. No. 567210; Right Panel at 5 mug/ml for one hour at room temperature. A three-step staining procedure that employs a Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 , Streptavidin-Horseradish Peroxidase Sav-HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The tissues were counterstained with hematoxylin. CD133 positive staining on syncytiotrophoblasts of chorionic villi Right Panel is indicated by the intense brown labeling. Original magnification: 20X. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD133 expression on human retinoblastoma cell line. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dotted line histogram or Purified Mouse Anti-Human CD133 antibody Cat. No. 567210; solid line histogram at 0.125 mug/test. The cells were washed and stained with PE Goat Anti-Mouse Ig Cat. No. 550589 . The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Immunohistochemical analysis of human CD133 expression by cells comprising human placenta tissue. Cold acetone-fixed frozen sections of human placenta were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse Anti-Human CD133 antibody Cat. No. 567210; Right Panel at 5 mug/ml for one hour at room temperature. A three-step staining procedure that employs a Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 , Streptavidin-Horseradish Peroxidase Sav-HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The tissues were counterstained with hematoxylin. CD133 positive staining on syncytiotrophoblasts of chorionic villi Right Panel is indicated by the intense brown labeling. Original magnification: 20X. Data shown on this Technical Data Sheet are not lot specific.