BD PharmingenTM Purified Mouse Anti-Human CD11c

Description

Analysis of CD11c Expression Left Panel - Flow cytometric analysis of CD11c expression on human peripheral blood leucocytes. Human whole blood was collected with heparin as the preferred anticoagulant rather than EDTA stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD11c antibody Cat. No. 565805; Right Plot . After washing, the cells were secondarily stained with PE Goat anti-Mouse Ig Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric contour plots showing the correlated expression of CD11c or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Right Panel - Three-color Immunofluorescense analysis of CD11c expression by cells in human tonsil. A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with Purified Mouse Anti-Human CD11c antibody followed by secondary staining with BD Horizon BV480 Goat Anti-Mouse Ig Cat. No. 564877, pseudocolored green . The section was thoroughly washed, then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427, pseudocolored red and Alexa Fluor 488 Mouse Anti-Human CD19 antibody Cat. No. 557697, pseudocolored blue . The slide was mounted with ProLong Gold. The images were captured using a standard epifluorescence microscope and merged. Original magnification, 20. Analysis of CD11c Expression Left Panel - Flow cytometric analysis of CD11c expression on human peripheral blood leucocytes. Human whole blood was collected with heparin as the preferred anticoagulant rather than EDTA stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD11c antibody Cat. No. 565805; Right Plot . After washing, the cells were secondarily stained with PE Goat anti-Mouse Ig Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric contour plots showing the correlated expression of CD11c or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Right Panel - Three-color Immunofluorescense analysis of CD11c expression by cells in human tonsil. A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with Purified Mouse Anti-Human CD11c antibody followed by secondary staining with BD Horizon BV480 Goat Anti-Mouse Ig Cat. No. 564877, pseudocolored green . The section was thoroughly washed, then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427, pseudocolored red and Alexa Fluor 488 Mouse Anti-Human CD19 antibody Cat. No. 557697, pseudocolored blue . The slide was mounted with ProLong Gold. The images were captured using a standard epifluorescence microscope and merged. Original magnification, 20. Show More Analysis of CD11c Expression Left Panel - Flow cytometric analysis of CD11c expression on human peripheral blood leucocytes. Human whole blood was collected with heparin as the preferred anticoagulant rather than EDTA stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD11c antibody Cat. No. 565805; Right Plot . After washing, the cells were secondarily stained with PE Goat anti-Mouse Ig Cat. No. 550589 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-parameter flow cytometric contour plots showing the correlated expression of CD11c or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Right Panel - Three-color Immunofluorescense analysis of CD11c expression by cells in human tonsil. A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1 PBS, and stained with Purified Mouse Anti-Human CD11c antibody followed by secondary staining with BD Horizon BV480 Goat Anti-Mouse Ig Cat. No. 564877, pseudocolored green . The section was thoroughly washed, then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427, pseudocolored red and Alexa Fluor 488 Mouse Anti-Human CD19 antibody Cat. No. 557697, pseudocolored blue . The slide was mounted with ProLong Gold. The images were captured using a standard epifluorescence microscope and merged. Original magnification, 20.