BD PharmingenTM Purified Mouse Anti-Human BCMA (CD269)

Description

Flow cytometric analysis of BCMA CD269 expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dashed line histograms or Purified Mouse Anti-BCMA CD269 antibody Cat. No. 570548; solid line histograms at 1 ug/test, followed by staining with either PE Goat Anti-Mouse Ig Cat. No. 550589 Left Panel or Biotin Goat Anti-Mouse Ig Cat. No. 553999 and then PE Streptavidin Cat. No. 554061 Right Panel . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing BCMA CD269 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of BCMA CD269 expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dashed line histograms or Purified Mouse Anti-BCMA CD269 antibody Cat. No. 570548; solid line histograms at 1 ug/test, followed by staining with either PE Goat Anti-Mouse Ig Cat. No. 550589 Left Panel or Biotin Goat Anti-Mouse Ig Cat. No. 553999 and then PE Streptavidin Cat. No. 554061 Right Panel . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing BCMA CD269 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of BCMA CD269 expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dashed line histograms or Purified Mouse Anti-BCMA CD269 antibody Cat. No. 570548; solid line histograms at 1 ug/test, followed by staining with either PE Goat Anti-Mouse Ig Cat. No. 550589 Left Panel or Biotin Goat Anti-Mouse Ig Cat. No. 553999 and then PE Streptavidin Cat. No. 554061 Right Panel . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing BCMA CD269 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of BCMA CD269 expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dashed line histograms or Purified Mouse Anti-BCMA CD269 antibody Cat. No. 570548; solid line histograms at 1 ug/test, followed by staining with either PE Goat Anti-Mouse Ig Cat. No. 550589 Left Panel or Biotin Goat Anti-Mouse Ig Cat. No. 553999 and then PE Streptavidin Cat. No. 554061 Right Panel . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing BCMA CD269 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of BCMA CD269 expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dashed line histograms or Purified Mouse Anti-BCMA CD269 antibody Cat. No. 570548; solid line histograms at 1 ug/test, followed by staining with either PE Goat Anti-Mouse Ig Cat. No. 550589 Left Panel or Biotin Goat Anti-Mouse Ig Cat. No. 553999 and then PE Streptavidin Cat. No. 554061 Right Panel . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing BCMA CD269 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of BCMA CD269 expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and labeled with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; dashed line histograms or Purified Mouse Anti-BCMA CD269 antibody Cat. No. 570548; solid line histograms at 1 ug/test, followed by staining with either PE Goat Anti-Mouse Ig Cat. No. 550589 Left Panel or Biotin Goat Anti-Mouse Ig Cat. No. 553999 and then PE Streptavidin Cat. No. 554061 Right Panel . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing BCMA CD269 expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.