BD PharmingenTM Purified Hamster Anti-Mouse CD80

Description

Expression of membrane CD80 by mouse splenocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141 and stained with either purified 16-10A1 mAb open histograms or purified hamster IgG isotype control mAb shaded histograms . Staining was detected with biotinylated mouse anti-hamster IgG Cat. No. 554010 followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a FACScan BD Biosciences, San Jose, CA . Expression of membrane CD80 by mouse splenocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141 and stained with either purified 16-10A1 mAb open histograms or purified hamster IgG isotype control mAb shaded histograms . Staining was detected with biotinylated mouse anti-hamster IgG Cat. No. 554010 followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a FACScan BD Biosciences, San Jose, CA . Expression of membrane CD80 by mouse splenocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141 and stained with either purified 16-10A1 mAb open histograms or purified hamster IgG isotype control mAb shaded histograms . Staining was detected with biotinylated mouse anti-hamster IgG Cat. No. 554010 followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a FACScan BD Biosciences, San Jose, CA . Show More Expression of membrane CD80 by mouse splenocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141 and stained with either purified 16-10A1 mAb open histograms or purified hamster IgG isotype control mAb shaded histograms . Staining was detected with biotinylated mouse anti-hamster IgG Cat. No. 554010 followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a FACScan BD Biosciences, San Jose, CA . Show More Expression of membrane CD80 by mouse splenocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141 and stained with either purified 16-10A1 mAb open histograms or purified hamster IgG isotype control mAb shaded histograms . Staining was detected with biotinylated mouse anti-hamster IgG Cat. No. 554010 followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a FACScan BD Biosciences, San Jose, CA . Expression of membrane CD80 by mouse splenocytes. Freshly isolated left panel or 72-hour LPS-stimulated BALB/c splenocytes right panel were pretreated with Mouse BD Fc Block anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141 and stained with either purified 16-10A1 mAb open histograms or purified hamster IgG isotype control mAb shaded histograms . Staining was detected with biotinylated mouse anti-hamster IgG Cat. No. 554010 followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a FACScan BD Biosciences, San Jose, CA .