BD PharmingenTM PerCP-CyTM5.5 Mouse anti-BrdU

Description

Flow cytometric analysis of DNA synthesis by TK-1 cells using PerCP-Cy5.5 anti-BrdU antibody. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the PerCP-Cy5.5 anti-BrdU antibody followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/ml Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System. Flow cytometric analysis of DNA synthesis by TK-1 cells using PerCP-Cy5.5 anti-BrdU antibody. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the PerCP-Cy5.5 anti-BrdU antibody followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/ml Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System. Show More Flow cytometric analysis of DNA synthesis by TK-1 cells using PerCP-Cy5.5 anti-BrdU antibody. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the PerCP-Cy5.5 anti-BrdU antibody followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/ml Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System.