BD PharmingenTM PE Rat Anti-Mouse Zbtb20

Description

Multicolor flow cytometric analysis of Zbtb20 expression in B1 or B2 subsets of mouse peritoneal B cells. Peritoneal cells were stained with APC-Cy7 Rat Anti-Mouse CD45R/B220 Cat. No. 552094 , BD Horizon BV421 Rat Anti-Mouse IgM Cat. No. 562595 , and BD Horizon PE-CF594 Rat Anti-Mouse CD23 Cat. No. 563986 antibodies. The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; dashed line histograms or PE Rat Anti-Mouse Zbtb20 antibody Cat. No. 565454; solid line histograms . The fluorescence histograms showing Zbtb20 expression or Ig Isotype control staining were derived from either B220+ IgM+ CD23- B1 cells; Top Right Plot or B220+ IgM low CD23+ B2 cells; Bottom Right Plot gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of Zbtb20 expression in B1 or B2 subsets of mouse peritoneal B cells. Peritoneal cells were stained with APC-Cy7 Rat Anti-Mouse CD45R/B220 Cat. No. 552094 , BD Horizon BV421 Rat Anti-Mouse IgM Cat. No. 562595 , and BD Horizon PE-CF594 Rat Anti-Mouse CD23 Cat. No. 563986 antibodies. The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; dashed line histograms or PE Rat Anti-Mouse Zbtb20 antibody Cat. No. 565454; solid line histograms . The fluorescence histograms showing Zbtb20 expression or Ig Isotype control staining were derived from either B220+ IgM+ CD23- B1 cells; Top Right Plot or B220+ IgM low CD23+ B2 cells; Bottom Right Plot gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of Zbtb20 expression in B1 or B2 subsets of mouse peritoneal B cells. Peritoneal cells were stained with APC-Cy7 Rat Anti-Mouse CD45R/B220 Cat. No. 552094 , BD Horizon BV421 Rat Anti-Mouse IgM Cat. No. 562595 , and BD Horizon PE-CF594 Rat Anti-Mouse CD23 Cat. No. 563986 antibodies. The cells were then washed, fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No. 554689; dashed line histograms or PE Rat Anti-Mouse Zbtb20 antibody Cat. No. 565454; solid line histograms . The fluorescence histograms showing Zbtb20 expression or Ig Isotype control staining were derived from either B220+ IgM+ CD23- B1 cells; Top Right Plot or B220+ IgM low CD23+ B2 cells; Bottom Right Plot gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.