BD PharmingenTM PE Rat Anti-Mouse MD-1

Description

Multicolor flow cytometric analysis of MD-1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leucocytes were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse MD-1 antibody Cat. No. 569972/569973; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MD-1 versus CD45R/B220 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of MD-1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leucocytes were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse MD-1 antibody Cat. No. 569972/569973; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MD-1 versus CD45R/B220 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of MD-1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leucocytes were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse MD-1 antibody Cat. No. 569972/569973; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MD-1 versus CD45R/B220 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of MD-1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leucocytes were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse MD-1 antibody Cat. No. 569972/569973; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MD-1 versus CD45R/B220 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of MD-1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leucocytes were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse MD-1 antibody Cat. No. 569972/569973; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MD-1 versus CD45R/B220 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of MD-1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leucocytes were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Rat Anti-Mouse MD-1 antibody Cat. No. 569972/569973; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MD-1 versus CD45R/B220 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.