BD PharmingenTM PE Rat Anti-Mouse DCIR1 (Clec4a2)

Description

Multicolor flow cytometric analysis of DCIR1 Clec4a2 expression on mouse splenic leucocyte populations. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; dashed line histograms or PE Rat Anti-Mouse DCIR1 Clec4a2 antibody Cat. No. 566810; solid line histograms at 0.5 mug/test and either 1 BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 and FITC Rat Anti-Mouse I-A/I-E Cat. No. 553623 antibodies to permit rectangular gating on conventional CD11c+ and I-A/I-E+ dendritic cells cDCs; Top Left Plot or 2 BD Horizon BUV395 Rat Anti-CD11b Cat. No. 563553 and FITC Rat Anti-Mouse Ly-6G Cat. No. 551460 antibodies to permit gating on CD11b+ and Ly-6G+ Neutrophils Bottom Left Plot as shown. Histograms showing DCIR1 Clec4a2 expression or Ig Isotype control staining for either gated cDCs Top Right Plot or Neutrophils Bottom Right Plot are displayed. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of DCIR1 Clec4a2 expression on mouse splenic leucocyte populations. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; dashed line histograms or PE Rat Anti-Mouse DCIR1 Clec4a2 antibody Cat. No. 566810; solid line histograms at 0.5 mug/test and either 1 BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 and FITC Rat Anti-Mouse I-A/I-E Cat. No. 553623 antibodies to permit rectangular gating on conventional CD11c+ and I-A/I-E+ dendritic cells cDCs; Top Left Plot or 2 BD Horizon BUV395 Rat Anti-CD11b Cat. No. 563553 and FITC Rat Anti-Mouse Ly-6G Cat. No. 551460 antibodies to permit gating on CD11b+ and Ly-6G+ Neutrophils Bottom Left Plot as shown. Histograms showing DCIR1 Clec4a2 expression or Ig Isotype control staining for either gated cDCs Top Right Plot or Neutrophils Bottom Right Plot are displayed. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of DCIR1 Clec4a2 expression on mouse splenic leucocyte populations. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; dashed line histograms or PE Rat Anti-Mouse DCIR1 Clec4a2 antibody Cat. No. 566810; solid line histograms at 0.5 mug/test and either 1 BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 and FITC Rat Anti-Mouse I-A/I-E Cat. No. 553623 antibodies to permit rectangular gating on conventional CD11c+ and I-A/I-E+ dendritic cells cDCs; Top Left Plot or 2 BD Horizon BUV395 Rat Anti-CD11b Cat. No. 563553 and FITC Rat Anti-Mouse Ly-6G Cat. No. 551460 antibodies to permit gating on CD11b+ and Ly-6G+ Neutrophils Bottom Left Plot as shown. Histograms showing DCIR1 Clec4a2 expression or Ig Isotype control staining for either gated cDCs Top Right Plot or Neutrophils Bottom Right Plot are displayed. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.