BD PharmingenTM PE Rat Anti-Human IL-10

Description

Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hr with LPS 1.0 ug/ml final concentration in the presence of GolgiStop Cat. No. 554724; 2 muM final concentration . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with 0.25 mug of PE Rat Anti-Human IL-10 antibody Cat. No. 554706/559330/562035 following BD Pharmingens staining protocol see image, left panel . The data reflect gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE Rat Anti-Human IL-10 was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 0.25 mug, Cat. No. 554611; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Rat Anti-Human IL-10 antibody 5.0 mug, Cat. No. 554705/554704; right panel prior to staining with the PE Rat Anti-Human IL-10 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking middle panel and unlabelled antibody right panel blocking specificity controls. Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hr with LPS 1.0 ug/ml final concentration in the presence of GolgiStop Cat. No. 554724; 2 muM final concentration . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with 0.25 mug of PE Rat Anti-Human IL-10 antibody Cat. No. 554706/559330/562035 following BD Pharmingens staining protocol see image, left panel . The data reflect gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE Rat Anti-Human IL-10 was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 0.25 mug, Cat. No. 554611; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Rat Anti-Human IL-10 antibody 5.0 mug, Cat. No. 554705/554704; right panel prior to staining with the PE Rat Anti-Human IL-10 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking middle panel and unlabelled antibody right panel blocking specificity controls. Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hr with LPS 1.0 ug/ml final concentration in the presence of GolgiStop Cat. No. 554724; 2 muM final concentration . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with 0.25 mug of PE Rat Anti-Human IL-10 antibody Cat. No. 554706/559330/562035 following BD Pharmingens staining protocol see image, left panel . The data reflect gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE Rat Anti-Human IL-10 was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 0.25 mug, Cat. No. 554611; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Rat Anti-Human IL-10 antibody 5.0 mug, Cat. No. 554705/554704; right panel prior to staining with the PE Rat Anti-Human IL-10 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking middle panel and unlabelled antibody right panel blocking specificity controls. Show More Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hr with LPS 1.0 ug/ml final concentration in the presence of GolgiStop Cat. No. 554724; 2 muM final concentration . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with 0.25 mug of PE Rat Anti-Human IL-10 antibody Cat. No. 554706/559330/562035 following BD Pharmingens staining protocol see image, left panel . The data reflect gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE Rat Anti-Human IL-10 was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 0.25 mug, Cat. No. 554611; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Rat Anti-Human IL-10 antibody 5.0 mug, Cat. No. 554705/554704; right panel prior to staining with the PE Rat Anti-Human IL-10 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking middle panel and unlabelled antibody right panel blocking specificity controls. Show More Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hr with LPS 1.0 ug/ml final concentration in the presence of GolgiStop Cat. No. 554724; 2 muM final concentration . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with 0.25 mug of PE Rat Anti-Human IL-10 antibody Cat. No. 554706/559330/562035 following BD Pharmingens staining protocol see image, left panel . The data reflect gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE Rat Anti-Human IL-10 was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 0.25 mug, Cat. No. 554611; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Rat Anti-Human IL-10 antibody 5.0 mug, Cat. No. 554705/554704; right panel prior to staining with the PE Rat Anti-Human IL-10 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking middle panel and unlabelled antibody right panel blocking specificity controls. Expression of IL-10 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 24 hr with LPS 1.0 ug/ml final concentration in the presence of GolgiStop Cat. No. 554724; 2 muM final concentration . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with 0.25 mug of PE Rat Anti-Human IL-10 antibody Cat. No. 554706/559330/562035 following BD Pharmingens staining protocol see image, left panel . The data reflect gating on monocytes, based on forward and side scatter. To demonstrate specificity of staining, the binding of PE Rat Anti-Human IL-10 was blocked by the preincubation of the conjugated antibody with recombinant human IL-10 0.25 mug, Cat. No. 554611; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Rat Anti-Human IL-10 antibody 5.0 mug, Cat. No. 554705/554704; right panel prior to staining with the PE Rat Anti-Human IL-10 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking middle panel and unlabelled antibody right panel blocking specificity controls.