BD PharmingenTM PE Rat Anti-Human CD120b

Description

Flow cytometric analysis of expression of cell surface TNFRII by lysed whole human blood. Whole human blood was blocked with normal polyclonal human IgG, stained with PE Rat Anti-Human CD120b 20 mul/1X106 cells, Cat No. 552418 and subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with PE Rat Anti-Human CD120b filled histogram is compared to staining obtained using PE anti-Rat IgG2b kappa, isotype control Cat. No. 553989; open histogram . The fluorescence histograms were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Flow cytometric analysis of expression of cell surface TNFRII by lysed whole human blood. Whole human blood was blocked with normal polyclonal human IgG, stained with PE Rat Anti-Human CD120b 20 mul/1X106 cells, Cat No. 552418 and subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with PE Rat Anti-Human CD120b filled histogram is compared to staining obtained using PE anti-Rat IgG2b kappa, isotype control Cat. No. 553989; open histogram . The fluorescence histograms were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Flow cytometric analysis of expression of cell surface TNFRII by lysed whole human blood. Whole human blood was blocked with normal polyclonal human IgG, stained with PE Rat Anti-Human CD120b 20 mul/1X106 cells, Cat No. 552418 and subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with PE Rat Anti-Human CD120b filled histogram is compared to staining obtained using PE anti-Rat IgG2b kappa, isotype control Cat. No. 553989; open histogram . The fluorescence histograms were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Show More Flow cytometric analysis of expression of cell surface TNFRII by lysed whole human blood. Whole human blood was blocked with normal polyclonal human IgG, stained with PE Rat Anti-Human CD120b 20 mul/1X106 cells, Cat No. 552418 and subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with PE Rat Anti-Human CD120b filled histogram is compared to staining obtained using PE anti-Rat IgG2b kappa, isotype control Cat. No. 553989; open histogram . The fluorescence histograms were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Show More Flow cytometric analysis of expression of cell surface TNFRII by lysed whole human blood. Whole human blood was blocked with normal polyclonal human IgG, stained with PE Rat Anti-Human CD120b 20 mul/1X106 cells, Cat No. 552418 and subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with PE Rat Anti-Human CD120b filled histogram is compared to staining obtained using PE anti-Rat IgG2b kappa, isotype control Cat. No. 553989; open histogram . The fluorescence histograms were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Flow cytometric analysis of expression of cell surface TNFRII by lysed whole human blood. Whole human blood was blocked with normal polyclonal human IgG, stained with PE Rat Anti-Human CD120b 20 mul/1X106 cells, Cat No. 552418 and subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with PE Rat Anti-Human CD120b filled histogram is compared to staining obtained using PE anti-Rat IgG2b kappa, isotype control Cat. No. 553989; open histogram . The fluorescence histograms were derived from gated events with the light scattering characteristics of viable lymphocytes left panel , monocytes center panel and granulocytes right panel . Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.