BD PharmingenTM PE Mouse anti-Oct3/4 (Human Isoform A)

Description

Analysis of Oct3/4 Isoform A on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix fixation buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control Dashed line, Cat. No. 554680 or PE Mouse anti-Oct3/4 Human Isoform A monoclonal antibody solid line . The histograms were derived from gated events based on light scattering characteristics of the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate. Analysis of Oct3/4 Isoform A on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix fixation buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control Dashed line, Cat. No. 554680 or PE Mouse anti-Oct3/4 Human Isoform A monoclonal antibody solid line . The histograms were derived from gated events based on light scattering characteristics of the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate. Show More Analysis of Oct3/4 Isoform A on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix fixation buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of either PE Mouse IgG1, kappa isotype control Dashed line, Cat. No. 554680 or PE Mouse anti-Oct3/4 Human Isoform A monoclonal antibody solid line . The histograms were derived from gated events based on light scattering characteristics of the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate.