BD PharmingenTM PE Mouse Anti-Mouse TCR Vgamma4

Description

Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Optibuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061 and with either PE Mouse IgG1, k Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569158/569159; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Optibuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061 and with either PE Mouse IgG1, k Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569158/569159; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Optibuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061 and with either PE Mouse IgG1, k Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569158/569159; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Optibuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061 and with either PE Mouse IgG1, k Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569158/569159; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Optibuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061 and with either PE Mouse IgG1, k Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569158/569159; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vgamma4 expression on viable Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Optibuild R718 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 751919 , FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061 and with either PE Mouse IgG1, k Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Mouse TCR Vgamma4 Cat. No. 569158/569159; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vgamma4 or Ig Isotype control staining versus TCRgammadelta was derived from gated events with the forward and side light- scatter characteristics of viable DAPI-negative CD3e-positive splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.