BD PharmingenTM PE Mouse Anti-Mouse alpha-GalCer:CD1d Complex

Description

Flow cytometric analysis of alpha-GalCer:CD1d complex expression on Mouse JAWSII cells. Cells from the Mouse JAWSII Immature dendritic cell; ATCC CRL-11904 cell line were either not Left Histograms or were Right Histograms loaded overnight with alpha-GalCer. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dotted line histograms or PE Mouse Anti-Mouse alpha-GalCer:CD1d complex antibody Cat. No. 569977/569978; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing alpha-GalCer:CD1d complex expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of alpha-GalCer:CD1d complex expression on Mouse JAWSII cells. Cells from the Mouse JAWSII Immature dendritic cell; ATCC CRL-11904 cell line were either not Left Histograms or were Right Histograms loaded overnight with alpha-GalCer. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dotted line histograms or PE Mouse Anti-Mouse alpha-GalCer:CD1d complex antibody Cat. No. 569977/569978; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing alpha-GalCer:CD1d complex expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of alpha-GalCer:CD1d complex expression on Mouse JAWSII cells. Cells from the Mouse JAWSII Immature dendritic cell; ATCC CRL-11904 cell line were either not Left Histograms or were Right Histograms loaded overnight with alpha-GalCer. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dotted line histograms or PE Mouse Anti-Mouse alpha-GalCer:CD1d complex antibody Cat. No. 569977/569978; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing alpha-GalCer:CD1d complex expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of alpha-GalCer:CD1d complex expression on Mouse JAWSII cells. Cells from the Mouse JAWSII Immature dendritic cell; ATCC CRL-11904 cell line were either not Left Histograms or were Right Histograms loaded overnight with alpha-GalCer. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dotted line histograms or PE Mouse Anti-Mouse alpha-GalCer:CD1d complex antibody Cat. No. 569977/569978; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing alpha-GalCer:CD1d complex expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of alpha-GalCer:CD1d complex expression on Mouse JAWSII cells. Cells from the Mouse JAWSII Immature dendritic cell; ATCC CRL-11904 cell line were either not Left Histograms or were Right Histograms loaded overnight with alpha-GalCer. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dotted line histograms or PE Mouse Anti-Mouse alpha-GalCer:CD1d complex antibody Cat. No. 569977/569978; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing alpha-GalCer:CD1d complex expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of alpha-GalCer:CD1d complex expression on Mouse JAWSII cells. Cells from the Mouse JAWSII Immature dendritic cell; ATCC CRL-11904 cell line were either not Left Histograms or were Right Histograms loaded overnight with alpha-GalCer. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Mouse IgG2a, kappa Isotype Control Cat. No. 553457; dotted line histograms or PE Mouse Anti-Mouse alpha-GalCer:CD1d complex antibody Cat. No. 569977/569978; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing alpha-GalCer:CD1d complex expression or Ig Isotype control staining were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.