BD PharmingenTM PE Mouse Anti-Human TCRalphabeta

Description

Two-color Flow Cytometric Analysis TCRalphabeta Expression on Human Peripheral Blood Lymphocytes Left Panel - Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The cells were washed and then stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 340409 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680, Left Plot or PE Mouse Anti-Human TCRalphabeta antibody Cat. No. 564728; Right Plot . Two-color flow cytometric contour plots showing the coexpression of CD19 vs TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Panel - Erythrocyte-lysed whole blood was similarly washed and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-Human TCRalphabeta antibody Right Plot . Two-color flow cytometric dot plots showing the coexpression of CD3 vs TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Two-color Flow Cytometric Analysis TCRalphabeta Expression on Human Peripheral Blood Lymphocytes Left Panel - Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The cells were washed and then stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 340409 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680, Left Plot or PE Mouse Anti-Human TCRalphabeta antibody Cat. No. 564728; Right Plot . Two-color flow cytometric contour plots showing the coexpression of CD19 vs TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Panel - Erythrocyte-lysed whole blood was similarly washed and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-Human TCRalphabeta antibody Right Plot . Two-color flow cytometric dot plots showing the coexpression of CD3 vs TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Two-color Flow Cytometric Analysis TCRalphabeta Expression on Human Peripheral Blood Lymphocytes Left Panel - Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The cells were washed and then stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 340409 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680, Left Plot or PE Mouse Anti-Human TCRalphabeta antibody Cat. No. 564728; Right Plot . Two-color flow cytometric contour plots showing the coexpression of CD19 vs TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Panel - Erythrocyte-lysed whole blood was similarly washed and then stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-Human TCRalphabeta antibody Right Plot . Two-color flow cytometric dot plots showing the coexpression of CD3 vs TCRalphabeta or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System.