BD PharmingenTM PE Mouse Anti-Human Ig kappa Light Chain

Description

Multicolor flow cytometric analysis of Ig kappa Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human Ig kappa Light Chain antibody Cat. No. 555792/562052; Right Panel . The bivariate pseudocolor density plots showing the correlated expression of Ig kappa Light Chain or Ig isotype control staining versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software. Multicolor flow cytometric analysis of Ig kappa Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human Ig kappa Light Chain antibody Cat. No. 555792/562052; Right Panel . The bivariate pseudocolor density plots showing the correlated expression of Ig kappa Light Chain or Ig isotype control staining versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software. Multicolor flow cytometric analysis of Ig kappa Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human Ig kappa Light Chain antibody Cat. No. 555792/562052; Right Panel . The bivariate pseudocolor density plots showing the correlated expression of Ig kappa Light Chain or Ig isotype control staining versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software. Show More Multicolor flow cytometric analysis of Ig kappa Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human Ig kappa Light Chain antibody Cat. No. 555792/562052; Right Panel . The bivariate pseudocolor density plots showing the correlated expression of Ig kappa Light Chain or Ig isotype control staining versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software. Show More Multicolor flow cytometric analysis of Ig kappa Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human Ig kappa Light Chain antibody Cat. No. 555792/562052; Right Panel . The bivariate pseudocolor density plots showing the correlated expression of Ig kappa Light Chain or Ig isotype control staining versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software. Multicolor flow cytometric analysis of Ig kappa Light Chain expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either PE Mouse IgG1, kappa Isotype Control Cat. No. 555749; Left Panel or PE Mouse Anti-Human Ig kappa Light Chain antibody Cat. No. 555792/562052; Right Panel . The bivariate pseudocolor density plots showing the correlated expression of Ig kappa Light Chain or Ig isotype control staining versus CD19 were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FloJo software.