BD PharmingenTM PE Mouse Anti-Human IFN-gamma

Description

Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334/561007 , fixed, permeabilized, and subsequently stained with 0.125 mug of PE Mouse Anti-Human IFN-gamma Cat. No. 554552/557074/559326/561056 . The binding of PE Mouse Anti-Human IFN-gamma was blocked by preincubation of cells with Purified Mouse Anti-Human IFN-gamma Cat. No. 554549, 5 mug; right panel . The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls. Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334/561007 , fixed, permeabilized, and subsequently stained with 0.125 mug of PE Mouse Anti-Human IFN-gamma Cat. No. 554552/557074/559326/561056 . The binding of PE Mouse Anti-Human IFN-gamma was blocked by preincubation of cells with Purified Mouse Anti-Human IFN-gamma Cat. No. 554549, 5 mug; right panel . The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls. Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334/561007 , fixed, permeabilized, and subsequently stained with 0.125 mug of PE Mouse Anti-Human IFN-gamma Cat. No. 554552/557074/559326/561056 . The binding of PE Mouse Anti-Human IFN-gamma was blocked by preincubation of cells with Purified Mouse Anti-Human IFN-gamma Cat. No. 554549, 5 mug; right panel . The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls. Show More Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334/561007 , fixed, permeabilized, and subsequently stained with 0.125 mug of PE Mouse Anti-Human IFN-gamma Cat. No. 554552/557074/559326/561056 . The binding of PE Mouse Anti-Human IFN-gamma was blocked by preincubation of cells with Purified Mouse Anti-Human IFN-gamma Cat. No. 554549, 5 mug; right panel . The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls. Show More Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334/561007 , fixed, permeabilized, and subsequently stained with 0.125 mug of PE Mouse Anti-Human IFN-gamma Cat. No. 554552/557074/559326/561056 . The binding of PE Mouse Anti-Human IFN-gamma was blocked by preincubation of cells with Purified Mouse Anti-Human IFN-gamma Cat. No. 554549, 5 mug; right panel . The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls. Expression of IFN-gamma by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334/561007 , fixed, permeabilized, and subsequently stained with 0.125 mug of PE Mouse Anti-Human IFN-gamma Cat. No. 554552/557074/559326/561056 . The binding of PE Mouse Anti-Human IFN-gamma was blocked by preincubation of cells with Purified Mouse Anti-Human IFN-gamma Cat. No. 554549, 5 mug; right panel . The quadrant markers for the bivariate dot plot were set based on the autofluorescence controls and verified using the unlabeled antibody and ligand blocking controls.