BD PharmingenTM PE Mouse Anti-Human CD215 (IL-15Ralpha)

Description

Multiparameter flow cytometric analysis of CD215 IL15Ralpha expression on human monocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium without left column or with right column lipopolysaccharide LPS, 100 ng/ml and Recombinant Human IFN-gamma Cat. No. 554617, 10 ng/ml for 20 hours at 37C. The cells were harvested, incubated with Human BD Fc Block Cat. No. 564220 , and then stained with APC Mouse Anti-Human CD14 Cat. No. 555399 and either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; top row or PE Mouse Anti-Human CD215 IL15Ralpha antibody Cat. No. 566589; bottom row . Two-color flow cytometric contour plots showing the correlated expression of CD215 or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa TM X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD215 IL15Ralpha expression on human monocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium without left column or with right column lipopolysaccharide LPS, 100 ng/ml and Recombinant Human IFN-gamma Cat. No. 554617, 10 ng/ml for 20 hours at 37C. The cells were harvested, incubated with Human BD Fc Block Cat. No. 564220 , and then stained with APC Mouse Anti-Human CD14 Cat. No. 555399 and either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; top row or PE Mouse Anti-Human CD215 IL15Ralpha antibody Cat. No. 566589; bottom row . Two-color flow cytometric contour plots showing the correlated expression of CD215 or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa TM X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD215 IL15Ralpha expression on human monocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium without left column or with right column lipopolysaccharide LPS, 100 ng/ml and Recombinant Human IFN-gamma Cat. No. 554617, 10 ng/ml for 20 hours at 37C. The cells were harvested, incubated with Human BD Fc Block Cat. No. 564220 , and then stained with APC Mouse Anti-Human CD14 Cat. No. 555399 and either PE Mouse IgG2b, kappa Isotype Control Cat. No. 555058; top row or PE Mouse Anti-Human CD215 IL15Ralpha antibody Cat. No. 566589; bottom row . Two-color flow cytometric contour plots showing the correlated expression of CD215 or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa TM X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.