BD PharmingenTM PE-CyTM7 Rat Anti-Mouse CD4

Description

Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and PE-Cy7 Rat Anti-Mouse CD4 Cat. No. 563933 antibodies. The two-color fluorescence dot plot shows the correlated expression patterns of CD4 versus CD3 for gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and PE-Cy7 Rat Anti-Mouse CD4 Cat. No. 563933 antibodies. The two-color fluorescence dot plot shows the correlated expression patterns of CD4 versus CD3 for gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two color flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and PE-Cy7 Rat Anti-Mouse CD4 Cat. No. 563933 antibodies. The two-color fluorescence dot plot shows the correlated expression patterns of CD4 versus CD3 for gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.