BD PharmingenTM PE-CyTM7 Mouse anti-Ki-67

Description

Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with PE-Cy7 Mouse Anti-Ki-67 antibody Cat. No. 561283 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with 4,6-Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 to stain double-stranded DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with PE-Cy7 Mouse Anti-Ki-67 antibody Cat. No. 561283 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with 4,6-Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 to stain double-stranded DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with PE-Cy7 Mouse Anti-Ki-67 antibody Cat. No. 561283 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with 4,6-Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 to stain double-stranded DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with PE-Cy7 Mouse Anti-Ki-67 antibody Cat. No. 561283 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with 4,6-Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 to stain double-stranded DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with PE-Cy7 Mouse Anti-Ki-67 antibody Cat. No. 561283 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with 4,6-Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 to stain double-stranded DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Ki-67 expression by proliferating Molt-4 and noncycling human peripheral blood mononuclear cells PBMC . Proliferating Molt-4 cells and noncycling PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with PE-Cy7 Mouse Anti-Ki-67 antibody Cat. No. 561283 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with 4,6-Diamidino-2-phenylindole dihydrochloride DAPI Sigma, Cat. No. D-9542 to stain double-stranded DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Molt-4 cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System.