BD PharmingenTM PE-Cy7 Hamster Anti-Mouse CD11c (Integrin alphaX)

Description

Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with C57BL/6 Mouse splenocytes followed by preincubation with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenic leukocytes were then stained with BD Horizon BV650 Rat Anti-Mouse I-A/I-E antibody Cat. No. 563415 and with either PE-Cy7 Hamster IgG1, kappa Isotype Control Cat. No. 552811; Left Plot; used as a representative Hamster IgG Isotype Control or PE-Cy7 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 570804/570880; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with C57BL/6 Mouse splenocytes followed by preincubation with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenic leukocytes were then stained with BD Horizon BV650 Rat Anti-Mouse I-A/I-E antibody Cat. No. 563415 and with either PE-Cy7 Hamster IgG1, kappa Isotype Control Cat. No. 552811; Left Plot; used as a representative Hamster IgG Isotype Control or PE-Cy7 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 570804/570880; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with C57BL/6 Mouse splenocytes followed by preincubation with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenic leukocytes were then stained with BD Horizon BV650 Rat Anti-Mouse I-A/I-E antibody Cat. No. 563415 and with either PE-Cy7 Hamster IgG1, kappa Isotype Control Cat. No. 552811; Left Plot; used as a representative Hamster IgG Isotype Control or PE-Cy7 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 570804/570880; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with C57BL/6 Mouse splenocytes followed by preincubation with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenic leukocytes were then stained with BD Horizon BV650 Rat Anti-Mouse I-A/I-E antibody Cat. No. 563415 and with either PE-Cy7 Hamster IgG1, kappa Isotype Control Cat. No. 552811; Left Plot; used as a representative Hamster IgG Isotype Control or PE-Cy7 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 570804/570880; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with C57BL/6 Mouse splenocytes followed by preincubation with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenic leukocytes were then stained with BD Horizon BV650 Rat Anti-Mouse I-A/I-E antibody Cat. No. 563415 and with either PE-Cy7 Hamster IgG1, kappa Isotype Control Cat. No. 552811; Left Plot; used as a representative Hamster IgG Isotype Control or PE-Cy7 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 570804/570880; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD11c Integrin alphaX expression on viable Mouse splenic leukocytes. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with C57BL/6 Mouse splenocytes followed by preincubation with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenic leukocytes were then stained with BD Horizon BV650 Rat Anti-Mouse I-A/I-E antibody Cat. No. 563415 and with either PE-Cy7 Hamster IgG1, kappa Isotype Control Cat. No. 552811; Left Plot; used as a representative Hamster IgG Isotype Control or PE-Cy7 Hamster Anti-Mouse CD11c Integrin alphaX antibody Cat. No. 570804/570880; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11c Integrin alphaX or Ig Isotype control staining versus I-A/I-E was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.