BD PharmingenTM Mouse Naive/Memory T Cell Panel

Description

Multicolor flow cytometric analysis of naive and memory CD4+ T cell subsets defined by the coexpressed levels of CD62L and CD44. Splenocytes from a young 4 weeks or older 9 weeks BALB/c mouse donor were stained for analysis using the FACSCalibur System Top Figures with a combination 5 mul each of PerCP-Cy5.5 Rat Anti-Mouse CD4, PE Rat Anti-Mouse CD44, and APC Rat Anti-Mouse CD62L. Splenocytes were also stained with APC-Cy7 Rat Anti-Mouse CD3 Molecular Complex, then analyzed on the BD LSR II and FACSCanto II systems Middle and Bottom Figures, respectively . Two-color flow cytometric dot plots showing the expression of CD62L and CD44 right dot plots were derived from CD4+ cells middle histograms with the light scattering characteristics of viable lymphocytes left FACSCalibur dotplot or viable CD3+ cells left LSR II and FACSCanto II dotplots . FSC vs SSC data from multiparameter analysis using the LSR II and FACSCanto II were also used for gating viable cells data not shown . The patterns for the coexpressed levels of CD62L and CD44 by CD4+ T cells from the same young BALB/c mouse donor were very similar as determined by the 3 different flow cytometer systems right dot plots . Interestingly, multicolor flow cytometric analysis of spleen cells from an older BALB/c mouse donor far right dot plot from FACSCanto II analysis revealed a significantly altered coexpression pattern of CD62L and CD44 by CD4+ T cells. Multicolor flow cytometric analysis of naive and memory CD4+ T cell subsets defined by the coexpressed levels of CD62L and CD44. Splenocytes from a young 4 weeks or older 9 weeks BALB/c mouse donor were stained for analysis using the FACSCalibur System Top Figures with a combination 5 mul each of PerCP-Cy5.5 Rat Anti-Mouse CD4, PE Rat Anti-Mouse CD44, and APC Rat Anti-Mouse CD62L. Splenocytes were also stained with APC-Cy7 Rat Anti-Mouse CD3 Molecular Complex, then analyzed on the BD LSR II and FACSCanto II systems Middle and Bottom Figures, respectively . Two-color flow cytometric dot plots showing the expression of CD62L and CD44 right dot plots were derived from CD4+ cells middle histograms with the light scattering characteristics of viable lymphocytes left FACSCalibur dotplot or viable CD3+ cells left LSR II and FACSCanto II dotplots . FSC vs SSC data from multiparameter analysis using the LSR II and FACSCanto II were also used for gating viable cells data not shown . The patterns for the coexpressed levels of CD62L and CD44 by CD4+ T cells from the same young BALB/c mouse donor were very similar as determined by the 3 different flow cytometer systems right dot plots . Interestingly, multicolor flow cytometric analysis of spleen cells from an older BALB/c mouse donor far right dot plot from FACSCanto II analysis revealed a significantly altered coexpression pattern of CD62L and CD44 by CD4+ T cells. Show More Multicolor flow cytometric analysis of naive and memory CD4+ T cell subsets defined by the coexpressed levels of CD62L and CD44. Splenocytes from a young 4 weeks or older 9 weeks BALB/c mouse donor were stained for analysis using the FACSCalibur System Top Figures with a combination 5 mul each of PerCP-Cy5.5 Rat Anti-Mouse CD4, PE Rat Anti-Mouse CD44, and APC Rat Anti-Mouse CD62L. Splenocytes were also stained with APC-Cy7 Rat Anti-Mouse CD3 Molecular Complex, then analyzed on the BD LSR II and FACSCanto II systems Middle and Bottom Figures, respectively . Two-color flow cytometric dot plots showing the expression of CD62L and CD44 right dot plots were derived from CD4+ cells middle histograms with the light scattering characteristics of viable lymphocytes left FACSCalibur dotplot or viable CD3+ cells left LSR II and FACSCanto II dotplots . FSC vs SSC data from multiparameter analysis using the LSR II and FACSCanto II were also used for gating viable cells data not shown . The patterns for the coexpressed levels of CD62L and CD44 by CD4+ T cells from the same young BALB/c mouse donor were very similar as determined by the 3 different flow cytometer systems right dot plots . Interestingly, multicolor flow cytometric analysis of spleen cells from an older BALB/c mouse donor far right dot plot from FACSCanto II analysis revealed a significantly altered coexpression pattern of CD62L and CD44 by CD4+ T cells.