BD PharmingenTM Human Regulatory T Cell Cocktail

Description

Three-color analysis of the expression of CD4, CD25, and CD127 on peripheral blood mononuclear cells PBMCs . PBMCs were stained with either PE-Cy7 Mouse IgG1 Isotype Control Cat. No. 557872/555909: data not shown or Human Regulatory T Cell Cocktail Cat. No. 560249 . The PBMCs were then fixed, lysed and permeabilized using BD Pharmingen Human FoxP3 Buffer Set Cat. No. 560098 and stained with PE conjugated anti-human FoxP3 monoclonal antibody Cat. No.560082 . During data analysis, lymphocytes were identified by light scatter profile and CD4 positive expression. The figure on the left represents the CD25 and CD127 expression profile of the CD4 postive cells. The panel on the right shows hFoxP3 expression on CD127dim/CD25bright T regulatory cells solid line and other T cells dashed line . Flow cytometry was performed on a BD FACSCanto. Three-color analysis of the expression of CD4, CD25, and CD127 on peripheral blood mononuclear cells PBMCs . PBMCs were stained with either PE-Cy7 Mouse IgG1 Isotype Control Cat. No. 557872/555909: data not shown or Human Regulatory T Cell Cocktail Cat. No. 560249 . The PBMCs were then fixed, lysed and permeabilized using BD Pharmingen Human FoxP3 Buffer Set Cat. No. 560098 and stained with PE conjugated anti-human FoxP3 monoclonal antibody Cat. No.560082 . During data analysis, lymphocytes were identified by light scatter profile and CD4 positive expression. The figure on the left represents the CD25 and CD127 expression profile of the CD4 postive cells. The panel on the right shows hFoxP3 expression on CD127dim/CD25bright T regulatory cells solid line and other T cells dashed line . Flow cytometry was performed on a BD FACSCanto. Three-color analysis of the expression of CD4, CD25, and CD127 on peripheral blood mononuclear cells PBMCs . PBMCs were stained with either PE-Cy7 Mouse IgG1 Isotype Control Cat. No. 557872/555909: data not shown or Human Regulatory T Cell Cocktail Cat. No. 560249 . The PBMCs were then fixed, lysed and permeabilized using BD Pharmingen Human FoxP3 Buffer Set Cat. No. 560098 and stained with PE conjugated anti-human FoxP3 monoclonal antibody Cat. No.560082 . During data analysis, lymphocytes were identified by light scatter profile and CD4 positive expression. The figure on the left represents the CD25 and CD127 expression profile of the CD4 postive cells. The panel on the right shows hFoxP3 expression on CD127dim/CD25bright T regulatory cells solid line and other T cells dashed line . Flow cytometry was performed on a BD FACSCanto. Show More Three-color analysis of the expression of CD4, CD25, and CD127 on peripheral blood mononuclear cells PBMCs . PBMCs were stained with either PE-Cy7 Mouse IgG1 Isotype Control Cat. No. 557872/555909: data not shown or Human Regulatory T Cell Cocktail Cat. No. 560249 . The PBMCs were then fixed, lysed and permeabilized using BD Pharmingen Human FoxP3 Buffer Set Cat. No. 560098 and stained with PE conjugated anti-human FoxP3 monoclonal antibody Cat. No.560082 . During data analysis, lymphocytes were identified by light scatter profile and CD4 positive expression. The figure on the left represents the CD25 and CD127 expression profile of the CD4 postive cells. The panel on the right shows hFoxP3 expression on CD127dim/CD25bright T regulatory cells solid line and other T cells dashed line . Flow cytometry was performed on a BD FACSCanto. Show More Three-color analysis of the expression of CD4, CD25, and CD127 on peripheral blood mononuclear cells PBMCs . PBMCs were stained with either PE-Cy7 Mouse IgG1 Isotype Control Cat. No. 557872/555909: data not shown or Human Regulatory T Cell Cocktail Cat. No. 560249 . The PBMCs were then fixed, lysed and permeabilized using BD Pharmingen Human FoxP3 Buffer Set Cat. No. 560098 and stained with PE conjugated anti-human FoxP3 monoclonal antibody Cat. No.560082 . During data analysis, lymphocytes were identified by light scatter profile and CD4 positive expression. The figure on the left represents the CD25 and CD127 expression profile of the CD4 postive cells. The panel on the right shows hFoxP3 expression on CD127dim/CD25bright T regulatory cells solid line and other T cells dashed line . Flow cytometry was performed on a BD FACSCanto. Three-color analysis of the expression of CD4, CD25, and CD127 on peripheral blood mononuclear cells PBMCs . PBMCs were stained with either PE-Cy7 Mouse IgG1 Isotype Control Cat. No. 557872/555909: data not shown or Human Regulatory T Cell Cocktail Cat. No. 560249 . The PBMCs were then fixed, lysed and permeabilized using BD Pharmingen Human FoxP3 Buffer Set Cat. No. 560098 and stained with PE conjugated anti-human FoxP3 monoclonal antibody Cat. No.560082 . During data analysis, lymphocytes were identified by light scatter profile and CD4 positive expression. The figure on the left represents the CD25 and CD127 expression profile of the CD4 postive cells. The panel on the right shows hFoxP3 expression on CD127dim/CD25bright T regulatory cells solid line and other T cells dashed line . Flow cytometry was performed on a BD FACSCanto.