BD PharmingenTM Hoechst 33342 Solution

Description

Multicolor immunofluorescence analysis of cell cycle of HeLa cells Left Panel . HeLa cells ATCC CCL-2 were stained with BD Pharmingen Cell Cycle Kit Cat. No. 558662 with Alexa Fluor 488 Mouse anti-BrdU pseudo-colored green , Alexa Fluor 647 Rat anti-Histone H3 pS28 pseudo-colored red , and Hoechst 33342 Cat. pseudo-colored blue . Co-staining of Hoechst 33342 and Histone H3 pS28 appears pink. The confocal image was captured using a 20x 0.75 NA objective with the BD Pathway 435 Bioimaging Analyzer and merged using BD Attovision Software. Flow cytometric analysis of HeLa cell DNA content Right panel . HeLa cells in log growth were dissociated from the growth medium using Cell Dissociation Buffer Life Technologies and resuspended in complete medium containing 10 mug/mL Hoechst 33342 for 60 minutes at 37C. Cells were pelleted by centrifugation, Hoechst-containing medium was removed, and cells were resuspend in PBS and analyzed for DNA content. Data was acquired on a BD LSRFortessa cell analyzer system. DNA content histogram was deconvoluted into G0/G1, S, and G2/M populations by FlowJo software TreeStar . Multicolor immunofluorescence analysis of cell cycle of HeLa cells Left Panel . HeLa cells ATCC CCL-2 were stained with BD Pharmingen Cell Cycle Kit Cat. No. 558662 with Alexa Fluor 488 Mouse anti-BrdU pseudo-colored green , Alexa Fluor 647 Rat anti-Histone H3 pS28 pseudo-colored red , and Hoechst 33342 Cat. pseudo-colored blue . Co-staining of Hoechst 33342 and Histone H3 pS28 appears pink. The confocal image was captured using a 20x 0.75 NA objective with the BD Pathway 435 Bioimaging Analyzer and merged using BD Attovision Software. Flow cytometric analysis of HeLa cell DNA content Right panel . HeLa cells in log growth were dissociated from the growth medium using Cell Dissociation Buffer Life Technologies and resuspended in complete medium containing 10 mug/mL Hoechst 33342 for 60 minutes at 37C. Cells were pelleted by centrifugation, Hoechst-containing medium was removed, and cells were resuspend in PBS and analyzed for DNA content. Data was acquired on a BD LSRFortessa cell analyzer system. DNA content histogram was deconvoluted into G0/G1, S, and G2/M populations by FlowJo software TreeStar . Multicolor immunofluorescence analysis of cell cycle of HeLa cells Left Panel . HeLa cells ATCC CCL-2 were stained with BD Pharmingen Cell Cycle Kit Cat. No. 558662 with Alexa Fluor 488 Mouse anti-BrdU pseudo-colored green , Alexa Fluor 647 Rat anti-Histone H3 pS28 pseudo-colored red , and Hoechst 33342 Cat. pseudo-colored blue . Co-staining of Hoechst 33342 and Histone H3 pS28 appears pink. The confocal image was captured using a 20x 0.75 NA objective with the BD Pathway 435 Bioimaging Analyzer and merged using BD Attovision Software. Flow cytometric analysis of HeLa cell DNA content Right panel . HeLa cells in log growth were dissociated from the growth medium using Cell Dissociation Buffer Life Technologies and resuspended in complete medium containing 10 mug/mL Hoechst 33342 for 60 minutes at 37C. Cells were pelleted by centrifugation, Hoechst-containing medium was removed, and cells were resuspend in PBS and analyzed for DNA content. Data was acquired on a BD LSRFortessa cell analyzer system. DNA content histogram was deconvoluted into G0/G1, S, and G2/M populations by FlowJo software TreeStar . Show More Multicolor immunofluorescence analysis of cell cycle of HeLa cells Left Panel . HeLa cells ATCC CCL-2 were stained with BD Pharmingen Cell Cycle Kit Cat. No. 558662 with Alexa Fluor 488 Mouse anti-BrdU pseudo-colored green , Alexa Fluor 647 Rat anti-Histone H3 pS28 pseudo-colored red , and Hoechst 33342 Cat. pseudo-colored blue . Co-staining of Hoechst 33342 and Histone H3 pS28 appears pink. The confocal image was captured using a 20x 0.75 NA objective with the BD Pathway 435 Bioimaging Analyzer and merged using BD Attovision Software. Flow cytometric analysis of HeLa cell DNA content Right panel . HeLa cells in log growth were dissociated from the growth medium using Cell Dissociation Buffer Life Technologies and resuspended in complete medium containing 10 mug/mL Hoechst 33342 for 60 minutes at 37C. Cells were pelleted by centrifugation, Hoechst-containing medium was removed, and cells were resuspend in PBS and analyzed for DNA content. Data was acquired on a BD LSRFortessa cell analyzer system. DNA content histogram was deconvoluted into G0/G1, S, and G2/M populations by FlowJo software TreeStar . Show More Multicolor immunofluorescence analysis of cell cycle of HeLa cells Left Panel . HeLa cells ATCC CCL-2 were stained with BD Pharmingen Cell Cycle Kit Cat. No. 558662 with Alexa Fluor 488 Mouse anti-BrdU pseudo-colored green , Alexa Fluor 647 Rat anti-Histone H3 pS28 pseudo-colored red , and Hoechst 33342 Cat. pseudo-colored blue . Co-staining of Hoechst 33342 and Histone H3 pS28 appears pink. The confocal image was captured using a 20x 0.75 NA objective with the BD Pathway 435 Bioimaging Analyzer and merged using BD Attovision Software. Flow cytometric analysis of HeLa cell DNA content Right panel . HeLa cells in log growth were dissociated from the growth medium using Cell Dissociation Buffer Life Technologies and resuspended in complete medium containing 10 mug/mL Hoechst 33342 for 60 minutes at 37C. Cells were pelleted by centrifugation, Hoechst-containing medium was removed, and cells were resuspend in PBS and analyzed for DNA content. Data was acquired on a BD LSRFortessa cell analyzer system. DNA content histogram was deconvoluted into G0/G1, S, and G2/M populations by FlowJo software TreeStar . Multicolor immunofluorescence analysis of cell cycle of HeLa cells Left Panel . HeLa cells ATCC CCL-2 were stained with BD Pharmingen Cell Cycle Kit Cat. No. 558662 with Alexa Fluor 488 Mouse anti-BrdU pseudo-colored green , Alexa Fluor 647 Rat anti-Histone H3 pS28 pseudo-colored red , and Hoechst 33342 Cat. pseudo-colored blue . Co-staining of Hoechst 33342 and Histone H3 pS28 appears pink. The confocal image was captured using a 20x 0.75 NA objective with the BD Pathway 435 Bioimaging Analyzer and merged using BD Attovision Software. Flow cytometric analysis of HeLa cell DNA content Right panel . HeLa cells in log growth were dissociated from the growth medium using Cell Dissociation Buffer Life Technologies and resuspended in complete medium containing 10 mug/mL Hoechst 33342 for 60 minutes at 37C. Cells were pelleted by centrifugation, Hoechst-containing medium was removed, and cells were resuspend in PBS and analyzed for DNA content. Data was acquired on a BD LSRFortessa cell analyzer system. DNA content histogram was deconvoluted into G0/G1, S, and G2/M populations by FlowJo software TreeStar .