BD PharmingenTM FITC Mouse Anti-Rat IgG1

Description

Detection of intracellular rat IgG1 in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described below using FITC-conjugated RG11/39.4 mAb filled histogram or the matched isotype control, FITC-conjugated 27-35 mAb open histogram, Cat. No. 555057 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Detection of intracellular rat IgG1 in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described below using FITC-conjugated RG11/39.4 mAb filled histogram or the matched isotype control, FITC-conjugated 27-35 mAb open histogram, Cat. No. 555057 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Detection of intracellular rat IgG1 in an antibody-secreting hybridoma cell line. Cells were fixed, permeabilized, and stained according to the method described below using FITC-conjugated RG11/39.4 mAb filled histogram or the matched isotype control, FITC-conjugated 27-35 mAb open histogram, Cat. No. 555057 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.