BD PharmingenTM FITC Mouse Anti-Rat Granulocytes

Description

Multiparameter flow cytometric analysis of Granulocytes expression on Lewis Rat bone marrow cells. Lewis Rat bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with either FITC Mouse IgM, kappa Isotype Control Cat. No. 553474; Left Plot or FITC Mouse Anti-Rat Granulocytes antibody Cat. No. 554907; Right Plot at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Granulocytes or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II Flow Cytometry System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Granulocytes expression on Lewis Rat bone marrow cells. Lewis Rat bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with either FITC Mouse IgM, kappa Isotype Control Cat. No. 553474; Left Plot or FITC Mouse Anti-Rat Granulocytes antibody Cat. No. 554907; Right Plot at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Granulocytes or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II Flow Cytometry System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Granulocytes expression on Lewis Rat bone marrow cells. Lewis Rat bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with either FITC Mouse IgM, kappa Isotype Control Cat. No. 553474; Left Plot or FITC Mouse Anti-Rat Granulocytes antibody Cat. No. 554907; Right Plot at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Granulocytes or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II Flow Cytometry System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of Granulocytes expression on Lewis Rat bone marrow cells. Lewis Rat bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with either FITC Mouse IgM, kappa Isotype Control Cat. No. 553474; Left Plot or FITC Mouse Anti-Rat Granulocytes antibody Cat. No. 554907; Right Plot at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Granulocytes or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II Flow Cytometry System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of Granulocytes expression on Lewis Rat bone marrow cells. Lewis Rat bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with either FITC Mouse IgM, kappa Isotype Control Cat. No. 553474; Left Plot or FITC Mouse Anti-Rat Granulocytes antibody Cat. No. 554907; Right Plot at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Granulocytes or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II Flow Cytometry System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Granulocytes expression on Lewis Rat bone marrow cells. Lewis Rat bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were then stained with either FITC Mouse IgM, kappa Isotype Control Cat. No. 553474; Left Plot or FITC Mouse Anti-Rat Granulocytes antibody Cat. No. 554907; Right Plot at 0.125 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Granulocytes or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II Flow Cytometry System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.