BD PharmingenTM FITC Mouse Anti-Human Granzyme A Reagent Set

Description

Profile of permeabilized peripheral blood lymphocytes analyzed by flow cytometry BDIS, San Jose, CA . Cells were collected, fixed, and permeabilized using the Cytofix/Cytoperm Kit Cat. No. 554715 for 20 minutes at room temperature RT , pelleted and washed twice with Perm/Wash Buffer. Cells were resuspended in Perm/Wash Buffer and stained with FITC anti-human Granzyme A clone CB9, Cat. No. 558905 or with an isotype control clone MOPC-21 for 20-30 minutes at RT in the dark. Cells were then washed once in Perm/Wash Buffer, resuspended in wash buffer and analyzed by flow cytometry. Profile of permeabilized peripheral blood lymphocytes analyzed by flow cytometry BDIS, San Jose, CA . Cells were collected, fixed, and permeabilized using the Cytofix/Cytoperm Kit Cat. No. 554715 for 20 minutes at room temperature RT , pelleted and washed twice with Perm/Wash Buffer. Cells were resuspended in Perm/Wash Buffer and stained with FITC anti-human Granzyme A clone CB9, Cat. No. 558905 or with an isotype control clone MOPC-21 for 20-30 minutes at RT in the dark. Cells were then washed once in Perm/Wash Buffer, resuspended in wash buffer and analyzed by flow cytometry. Show More Profile of permeabilized peripheral blood lymphocytes analyzed by flow cytometry BDIS, San Jose, CA . Cells were collected, fixed, and permeabilized using the Cytofix/Cytoperm Kit Cat. No. 554715 for 20 minutes at room temperature RT , pelleted and washed twice with Perm/Wash Buffer. Cells were resuspended in Perm/Wash Buffer and stained with FITC anti-human Granzyme A clone CB9, Cat. No. 558905 or with an isotype control clone MOPC-21 for 20-30 minutes at RT in the dark. Cells were then washed once in Perm/Wash Buffer, resuspended in wash buffer and analyzed by flow cytometry.