BD PharmingenTM FITC Hamster Anti-Mouse Bcl-2 Set

Description

Flow cytometric analysis of Bcl-2 expression on permeabilized M1 mouse myelobast cells. M1 mouse myeloblast cells ATCC TIB-191 were permeabilized with Fixation and Permeabilization Solution Cat. No. 554722 and washed twice with Perm/Wash Buffer Cat. No. 554723 . Permeabilized cells were stained with either FITC Hamster Anti-Mouse Bcl-2 Cat. No. 554221, component51-15024X, filled histogram or FITC Hamster IgG Isotype Control Cat. No. 554221, component 51-66994X, open histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan system. Flow cytometric analysis of Bcl-2 expression on permeabilized M1 mouse myelobast cells. M1 mouse myeloblast cells ATCC TIB-191 were permeabilized with Fixation and Permeabilization Solution Cat. No. 554722 and washed twice with Perm/Wash Buffer Cat. No. 554723 . Permeabilized cells were stained with either FITC Hamster Anti-Mouse Bcl-2 Cat. No. 554221, component51-15024X, filled histogram or FITC Hamster IgG Isotype Control Cat. No. 554221, component 51-66994X, open histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan system. Show More Flow cytometric analysis of Bcl-2 expression on permeabilized M1 mouse myelobast cells. M1 mouse myeloblast cells ATCC TIB-191 were permeabilized with Fixation and Permeabilization Solution Cat. No. 554722 and washed twice with Perm/Wash Buffer Cat. No. 554723 . Permeabilized cells were stained with either FITC Hamster Anti-Mouse Bcl-2 Cat. No. 554221, component51-15024X, filled histogram or FITC Hamster IgG Isotype Control Cat. No. 554221, component 51-66994X, open histogram . Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan system.