BD PharmingenTM Biotin Rat Anti-Mouse CD115 (CSF-1R)

Description

Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either Biotin Rat IgG1, kappa Isotype Control Cat. No. 553923; Left Plot or Biotin Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567598; Right Plot at 0.25 mug/test, followed by PE Streptavidin Cat. No. 554061 . A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either Biotin Rat IgG1, kappa Isotype Control Cat. No. 553923; Left Plot or Biotin Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567598; Right Plot at 0.25 mug/test, followed by PE Streptavidin Cat. No. 554061 . A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either Biotin Rat IgG1, kappa Isotype Control Cat. No. 553923; Left Plot or Biotin Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567598; Right Plot at 0.25 mug/test, followed by PE Streptavidin Cat. No. 554061 . A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either Biotin Rat IgG1, kappa Isotype Control Cat. No. 553923; Left Plot or Biotin Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567598; Right Plot at 0.25 mug/test, followed by PE Streptavidin Cat. No. 554061 . A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either Biotin Rat IgG1, kappa Isotype Control Cat. No. 553923; Left Plot or Biotin Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567598; Right Plot at 0.25 mug/test, followed by PE Streptavidin Cat. No. 554061 . A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either Biotin Rat IgG1, kappa Isotype Control Cat. No. 553923; Left Plot or Biotin Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567598; Right Plot at 0.25 mug/test, followed by PE Streptavidin Cat. No. 554061 . A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.