BD PharmingenTM Biotin Mouse Anti-Human HLA-A2

Description

Multiparameter flow cytometric analysis of HLA-A2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415; Left Plot or Biotin Mouse Anti-Human HLA-A2 antibody Cat. No. 567728; Right Plot at 1.0 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of HLA-A2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415; Left Plot or Biotin Mouse Anti-Human HLA-A2 antibody Cat. No. 567728; Right Plot at 1.0 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of HLA-A2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415; Left Plot or Biotin Mouse Anti-Human HLA-A2 antibody Cat. No. 567728; Right Plot at 1.0 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of HLA-A2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415; Left Plot or Biotin Mouse Anti-Human HLA-A2 antibody Cat. No. 567728; Right Plot at 1.0 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of HLA-A2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415; Left Plot or Biotin Mouse Anti-Human HLA-A2 antibody Cat. No. 567728; Right Plot at 1.0 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of HLA-A2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415; Left Plot or Biotin Mouse Anti-Human HLA-A2 antibody Cat. No. 567728; Right Plot at 1.0 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.