BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human SCIMP

Description

Two-color flow cytometric analysis of SCIMP expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human HLA-DR, DP, DQ Cat. No. 555558/550853/562008 and then washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . After the cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No 554723 , they were stained with either Alexa Fluor647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365, Left Plot or Alexa Fluor 647 Mouse Anti-Human SCIMP antibody Cat. No. 568780/568955; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of SCIMP or Ig Isotype control staining versus HLA-DR, DP, DQ was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of SCIMP expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human HLA-DR, DP, DQ Cat. No. 555558/550853/562008 and then washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . After the cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No 554723 , they were stained with either Alexa Fluor647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365, Left Plot or Alexa Fluor 647 Mouse Anti-Human SCIMP antibody Cat. No. 568780/568955; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of SCIMP or Ig Isotype control staining versus HLA-DR, DP, DQ was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of SCIMP expression in Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with FITC Mouse Anti-Human HLA-DR, DP, DQ Cat. No. 555558/550853/562008 and then washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . After the cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No 554723 , they were stained with either Alexa Fluor647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365, Left Plot or Alexa Fluor 647 Mouse Anti-Human SCIMP antibody Cat. No. 568780/568955; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of SCIMP or Ig Isotype control staining versus HLA-DR, DP, DQ was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.