BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human HLA-E

Description

Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either Alexa Fluor 488 IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human HLA-E antibody Cat. No. 567416/567417; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A two-parameter pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either Alexa Fluor 488 IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human HLA-E antibody Cat. No. 567416/567417; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A two-parameter pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of HLA-E expression on human peripheral blood leucocyte populations. Human whole blood was stained with either Alexa Fluor 488 IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human HLA-E antibody Cat. No. 567416/567417; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A two-parameter pseudocolor density plot showing the correlated expression of HLA-E or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.