BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human CD133

Description

CD133 expression on retinoblastoma cell line and peripheral blood mononuclear cells PBMC Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Cat. No. 565383; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Cat. No. 567907/567908 solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon CD34 APC-R700 antibody Cat. No. 659114 and either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Left Plot or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Left Plot or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Right Plot at 0.5 mug/test followed by Cell Viability 7-AAD Solution addition before analysis. The pseudocolor density plot showing the correlated expression of CD45 versus CD133 or Ig Isotype control staining was similarly derived for viable PBL. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. CD133 expression on retinoblastoma cell line and peripheral blood mononuclear cells PBMC Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Cat. No. 565383; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Cat. No. 567907/567908 solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon CD34 APC-R700 antibody Cat. No. 659114 and either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Left Plot or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Left Plot or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Right Plot at 0.5 mug/test followed by Cell Viability 7-AAD Solution addition before analysis. The pseudocolor density plot showing the correlated expression of CD45 versus CD133 or Ig Isotype control staining was similarly derived for viable PBL. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More CD133 expression on retinoblastoma cell line and peripheral blood mononuclear cells PBMC Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Cat. No. 565383; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Cat. No. 567907/567908 solid line histogram at 0.5 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon CD34 APC-R700 antibody Cat. No. 659114 and either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Left Plot or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Left Plot or Alexa Fluor 488 Mouse Anti-Human CD133 antibody Right Plot at 0.5 mug/test followed by Cell Viability 7-AAD Solution addition before analysis. The pseudocolor density plot showing the correlated expression of CD45 versus CD133 or Ig Isotype control staining was similarly derived for viable PBL. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.