BD PharmingenTM Alexa Fluor 647 Rabbit Anti-Active Caspase-3

Description

Flow cytometric analysis of apoptotic and non-apoptotic populations for Active Caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated shaded or were treated with 4-12 muM of camptothecin Sigma-Aldrich Cat. No. C9911 for 4-6 hr to induce apoptosis unshaded . Cells were washed once in 1X PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 followed by staining with the Alexa Fluor 647 Rabbit Anti-Active Caspase-3 antibody. Histograms were derived from gated events based on light scattering characteristics for Jurkat cells. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of apoptotic and non-apoptotic populations for Active Caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated shaded or were treated with 4-12 muM of camptothecin Sigma-Aldrich Cat. No. C9911 for 4-6 hr to induce apoptosis unshaded . Cells were washed once in 1X PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 followed by staining with the Alexa Fluor 647 Rabbit Anti-Active Caspase-3 antibody. Histograms were derived from gated events based on light scattering characteristics for Jurkat cells. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis of apoptotic and non-apoptotic populations for Active Caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated shaded or were treated with 4-12 muM of camptothecin Sigma-Aldrich Cat. No. C9911 for 4-6 hr to induce apoptosis unshaded . Cells were washed once in 1X PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 followed by staining with the Alexa Fluor 647 Rabbit Anti-Active Caspase-3 antibody. Histograms were derived from gated events based on light scattering characteristics for Jurkat cells. Flow cytometry was performed on a BD LSR II flow cytometry system.