BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human CD4

Description

Multiparameter flow cytometric analysis of CD4 expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 566681/566682; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter pseudocolor dot plots showing the correlated expression of CD4 or Ig Isotype control staining versus side light-scatter SSC signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Multiparameter flow cytometric analysis of CD4 expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 566681/566682; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter pseudocolor dot plots showing the correlated expression of CD4 or Ig Isotype control staining versus side light-scatter SSC signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Multiparameter flow cytometric analysis of CD4 expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD4 antibody Cat. No. 566681/566682; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-parameter pseudocolor dot plots showing the correlated expression of CD4 or Ig Isotype control staining versus side light-scatter SSC signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System.