BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human CD258 (LIGHT)

Description

Two-color flow cytometric analysis of CD258 LIGHT expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated 36 hr with Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139; 1 ng/ml and Ionomycin Sigma Cat. No. I-0634; 1 mug/ml . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD8 antibody Cat. No. 557696/ 557704 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Alexa Fluor 647 Mouse Anti-Human CD258 LIGHT antibody Cat. No. 564374; Right Panel . The two-color flow cytometric contour plots showing the correlated expression patterns of CD8 versus CD258 LIGHT or Ig Isotype control staining were generated for gated events with the forward and side-light scattering characteristics of viable activated lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Two-color flow cytometric analysis of CD258 LIGHT expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated 36 hr with Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139; 1 ng/ml and Ionomycin Sigma Cat. No. I-0634; 1 mug/ml . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD8 antibody Cat. No. 557696/ 557704 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Alexa Fluor 647 Mouse Anti-Human CD258 LIGHT antibody Cat. No. 564374; Right Panel . The two-color flow cytometric contour plots showing the correlated expression patterns of CD8 versus CD258 LIGHT or Ig Isotype control staining were generated for gated events with the forward and side-light scattering characteristics of viable activated lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Two-color flow cytometric analysis of CD258 LIGHT expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated 36 hr with Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139; 1 ng/ml and Ionomycin Sigma Cat. No. I-0634; 1 mug/ml . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD8 antibody Cat. No. 557696/ 557704 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Alexa Fluor 647 Mouse Anti-Human CD258 LIGHT antibody Cat. No. 564374; Right Panel . The two-color flow cytometric contour plots showing the correlated expression patterns of CD8 versus CD258 LIGHT or Ig Isotype control staining were generated for gated events with the forward and side-light scattering characteristics of viable activated lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Show More Two-color flow cytometric analysis of CD258 LIGHT expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated 36 hr with Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139; 1 ng/ml and Ionomycin Sigma Cat. No. I-0634; 1 mug/ml . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD8 antibody Cat. No. 557696/ 557704 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Alexa Fluor 647 Mouse Anti-Human CD258 LIGHT antibody Cat. No. 564374; Right Panel . The two-color flow cytometric contour plots showing the correlated expression patterns of CD8 versus CD258 LIGHT or Ig Isotype control staining were generated for gated events with the forward and side-light scattering characteristics of viable activated lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Show More Two-color flow cytometric analysis of CD258 LIGHT expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated 36 hr with Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139; 1 ng/ml and Ionomycin Sigma Cat. No. I-0634; 1 mug/ml . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD8 antibody Cat. No. 557696/ 557704 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Alexa Fluor 647 Mouse Anti-Human CD258 LIGHT antibody Cat. No. 564374; Right Panel . The two-color flow cytometric contour plots showing the correlated expression patterns of CD8 versus CD258 LIGHT or Ig Isotype control staining were generated for gated events with the forward and side-light scattering characteristics of viable activated lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Two-color flow cytometric analysis of CD258 LIGHT expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated 36 hr with Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich, Cat. No. P-8139; 1 ng/ml and Ionomycin Sigma Cat. No. I-0634; 1 mug/ml . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD8 antibody Cat. No. 557696/ 557704 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Alexa Fluor 647 Mouse Anti-Human CD258 LIGHT antibody Cat. No. 564374; Right Panel . The two-color flow cytometric contour plots showing the correlated expression patterns of CD8 versus CD258 LIGHT or Ig Isotype control staining were generated for gated events with the forward and side-light scattering characteristics of viable activated lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System.