BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human CD148

Description

Analysis of Human CD148 Expression Left Panel - Two-parameter flow cytometric analysis of CD148 expression on human peripheral blood leucocytes. Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The cells were washed and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714, Left Plot or Alexa Fluor 647 Mouse Anti-Human CD148 antibody Cat. No. 564732; Right Plot . Flow cytometric contour plots showing the expression profile of CD148 or Ig isotype control staining versus Side Scattered-Light signals SSC were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Panel - Two-color flow cytometric analysis of CD148 expression on human peripheral blood lymphocytes. Erythrocyte-lysed whole blood was similarly washed and then stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 340409 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Left Plot or Alexa Fluor 647 Mouse Anti-Human CD148 antibody Right Plot . Two-color contour plots showing the coexpressed levels of CD19 versus CD148 or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Analysis of Human CD148 Expression Left Panel - Two-parameter flow cytometric analysis of CD148 expression on human peripheral blood leucocytes. Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The cells were washed and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714, Left Plot or Alexa Fluor 647 Mouse Anti-Human CD148 antibody Cat. No. 564732; Right Plot . Flow cytometric contour plots showing the expression profile of CD148 or Ig isotype control staining versus Side Scattered-Light signals SSC were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Panel - Two-color flow cytometric analysis of CD148 expression on human peripheral blood lymphocytes. Erythrocyte-lysed whole blood was similarly washed and then stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 340409 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Left Plot or Alexa Fluor 647 Mouse Anti-Human CD148 antibody Right Plot . Two-color contour plots showing the coexpressed levels of CD19 versus CD148 or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Analysis of Human CD148 Expression Left Panel - Two-parameter flow cytometric analysis of CD148 expression on human peripheral blood leucocytes. Whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The cells were washed and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714, Left Plot or Alexa Fluor 647 Mouse Anti-Human CD148 antibody Cat. No. 564732; Right Plot . Flow cytometric contour plots showing the expression profile of CD148 or Ig isotype control staining versus Side Scattered-Light signals SSC were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Panel - Two-color flow cytometric analysis of CD148 expression on human peripheral blood lymphocytes. Erythrocyte-lysed whole blood was similarly washed and then stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 340409 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Left Plot or Alexa Fluor 647 Mouse Anti-Human CD148 antibody Right Plot . Two-color contour plots showing the coexpressed levels of CD19 versus CD148 or Ig isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System.