BD PharmingenTM Alexa Fluor 647 Mouse anti-E-Cadherin

Description

Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Show More Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Show More Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Show More Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Left Image: Immunofluorescent staining of mouse small intestine. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded section was stained with Alexa Fluor 647 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure for tissue sections. The image was captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Image: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure .