BD PharmingenTM Alexa Fluor 647 Mouse anti-CDX-2

Description

Analysis of CDX-2 on human colorectal adenocarcinoma cell line DLD-1. DLD-1 cells ATCC CCL-221 were fixed BD Cytofix Cat. No. 554655 and permeabilized with the BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , then stained with Alexa Fluor 647 Mouse anti-CDX-2 solid line or Alexa Fluor 647 Mouse IgG1 Isotype Control clone MOPC-21, Cat. No. 557732 dashed line . This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of CDX-2 on spontaneously differentiated embryonic stem cells. Human embryonic stem cells H9; WiCell, Madison, WI were spontaneously differentiated in a 6-well plate for 3 weeks. Cells were fixed with BD Cytofix Cat. No. 554655 and permeabilized with the BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , then stained with Alexa Fluor 647 Mouse anti-CDX-2 and PE Mouse anti-Oct3/4 clone 40/Oct-3, Cat. No. 560186 . Analysis of CDX-2 on human colorectal adenocarcinoma cell line DLD-1. DLD-1 cells ATCC CCL-221 were fixed BD Cytofix Cat. No. 554655 and permeabilized with the BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , then stained with Alexa Fluor 647 Mouse anti-CDX-2 solid line or Alexa Fluor 647 Mouse IgG1 Isotype Control clone MOPC-21, Cat. No. 557732 dashed line . This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Analysis of CDX-2 on spontaneously differentiated embryonic stem cells. Human embryonic stem cells H9; WiCell, Madison, WI were spontaneously differentiated in a 6-well plate for 3 weeks. Cells were fixed with BD Cytofix Cat. No. 554655 and permeabilized with the BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , then stained with Alexa Fluor 647 Mouse anti-CDX-2 and PE Mouse anti-Oct3/4 clone 40/Oct-3, Cat. No. 560186 . Show More Analysis of CDX-2 on human colorectal adenocarcinoma cell line DLD-1. DLD-1 cells ATCC CCL-221 were fixed BD Cytofix Cat. No. 554655 and permeabilized with the BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , then stained with Alexa Fluor 647 Mouse anti-CDX-2 solid line or Alexa Fluor 647 Mouse IgG1 Isotype Control clone MOPC-21, Cat. No. 557732 dashed line . This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of CDX-2 on spontaneously differentiated embryonic stem cells. Human embryonic stem cells H9; WiCell, Madison, WI were spontaneously differentiated in a 6-well plate for 3 weeks. Cells were fixed with BD Cytofix Cat. No. 554655 and permeabilized with the BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , then stained with Alexa Fluor 647 Mouse anti-CDX-2 and PE Mouse anti-Oct3/4 clone 40/Oct-3, Cat. No. 560186 .