BD PharmingenTM Alexa Fluor 488 Mouse anti-Oct3/4

Description

Immunofluorescent staining of human ES cell line. H9 cells WiCell, Madison, WI were grown on mouse epithelial feeder cells in a BD Falcon 96-well Imaging Plate Cat. No. 353219 . After overnight culture, the cells were fixed, permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 488 Mouse anti-Oct3/4 pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Bioimager System with a 10x objective and merged using BD Attovision software. This antibody also stained human EC NCCIT ATCC, Cat. No. CRL-2073 , mouse EC F9 ATCC, Cat. No. CRL-1720 , and mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells. It also works with Triton X-100 and methanol permeabilization see Recommended Assay Procedure . Immunofluorescent staining of human ES cell line. H9 cells WiCell, Madison, WI were grown on mouse epithelial feeder cells in a BD Falcon 96-well Imaging Plate Cat. No. 353219 . After overnight culture, the cells were fixed, permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 488 Mouse anti-Oct3/4 pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Bioimager System with a 10x objective and merged using BD Attovision software. This antibody also stained human EC NCCIT ATCC, Cat. No. CRL-2073 , mouse EC F9 ATCC, Cat. No. CRL-1720 , and mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells. It also works with Triton X-100 and methanol permeabilization see Recommended Assay Procedure . Show More Immunofluorescent staining of human ES cell line. H9 cells WiCell, Madison, WI were grown on mouse epithelial feeder cells in a BD Falcon 96-well Imaging Plate Cat. No. 353219 . After overnight culture, the cells were fixed, permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 488 Mouse anti-Oct3/4 pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Bioimager System with a 10x objective and merged using BD Attovision software. This antibody also stained human EC NCCIT ATCC, Cat. No. CRL-2073 , mouse EC F9 ATCC, Cat. No. CRL-1720 , and mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells. It also works with Triton X-100 and methanol permeabilization see Recommended Assay Procedure .