BD PharmingenTM Alexa Fluor 488 Mouse Anti-Human Perforin

Description

Flow cytometric analysis of perforin expression in human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Cat. No. 558716; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human Perforin antibody Cat. No. 563764; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of perforin expression in human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Cat. No. 558716; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human Perforin antibody Cat. No. 563764; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of perforin expression in human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 488 Mouse IgG2b, kappa Isotype Control Cat. No. 558716; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human Perforin antibody Cat. No. 563764; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.