BD OptiBuildTM BV421 Mouse Anti-Human ULBP-2/5/6

Description

Flow cytometric analysis of ULBP-2/5/6 expression on human acute T cell leukemia and fibrosarcoma cells. Left Plot: Jurkat cells ATCC TIB-152 were stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. Right Plot: HT1080 cells ATCC CCL-121 were detached using Cell Dissociation Buffer Gibco 13151014 , and then stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. The fluorescence histograms showing ULBP-2/5/6 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of ULBP-2/5/6 expression on human acute T cell leukemia and fibrosarcoma cells. Left Plot: Jurkat cells ATCC TIB-152 were stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. Right Plot: HT1080 cells ATCC CCL-121 were detached using Cell Dissociation Buffer Gibco 13151014 , and then stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. The fluorescence histograms showing ULBP-2/5/6 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of ULBP-2/5/6 expression on human acute T cell leukemia and fibrosarcoma cells. Left Plot: Jurkat cells ATCC TIB-152 were stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. Right Plot: HT1080 cells ATCC CCL-121 were detached using Cell Dissociation Buffer Gibco 13151014 , and then stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. The fluorescence histograms showing ULBP-2/5/6 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Show More Flow cytometric analysis of ULBP-2/5/6 expression on human acute T cell leukemia and fibrosarcoma cells. Left Plot: Jurkat cells ATCC TIB-152 were stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. Right Plot: HT1080 cells ATCC CCL-121 were detached using Cell Dissociation Buffer Gibco 13151014 , and then stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. The fluorescence histograms showing ULBP-2/5/6 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Show More Flow cytometric analysis of ULBP-2/5/6 expression on human acute T cell leukemia and fibrosarcoma cells. Left Plot: Jurkat cells ATCC TIB-152 were stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. Right Plot: HT1080 cells ATCC CCL-121 were detached using Cell Dissociation Buffer Gibco 13151014 , and then stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. The fluorescence histograms showing ULBP-2/5/6 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of ULBP-2/5/6 expression on human acute T cell leukemia and fibrosarcoma cells. Left Plot: Jurkat cells ATCC TIB-152 were stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. Right Plot: HT1080 cells ATCC CCL-121 were detached using Cell Dissociation Buffer Gibco 13151014 , and then stained with either BD OptiBuild BV421 Mouse Anti-Human ULBP-2/5/6 Cat. No. 748128, solid line histogram or BD Horizon BV421 BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; dashed line histogram at 0.5 mug/test. The fluorescence histograms showing ULBP-2/5/6 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot.