BD IMagTM Human Naive CD4 T Cell Enrichment Set - DM

Description

Enrichment of naive CD4+ T cells from human peripheral blood. PBMC were labeled with the BD IMag Human Naive CD4+ T cell Enrichment Set - DM Cat. No. 558521 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. In panels A and B, cells were stained with a cocktail consisting of PE Anti-Human CD8 Cat. No. 555367 , CD11b Cat. No. 555388 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD36 Cat. No. 555455 , CD45RO Cat. No. 555493 , CD56 Cat. No. 555516 , CD123 Cat. No. 555644 , CD235a Cat. No. 555570 , and gammadelta TCR Cat No. 555717 to detect non-naive CD4+ cells, and an APC Human CD45RA Cat. No. 550855 to detect naive cells. For panels C and D, cells were stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349 and FITC Mouse Anti-Human CD45RA antibody Cat. No. 555488 to show the percentage of naive CD4+ T cells. Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Refer to the Enrichment Flow Chart to identify the cell populations in this figure. The percentage of naive CD4+ T cells is indicated in each panel. Panels A and C show unseparated PBMCs while panels B and D show the twice-enriched fraction after three 6-minute magnetic separations with an additional 3-10 minute separation. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Enrichment of naive CD4+ T cells from human peripheral blood. PBMC were labeled with the BD IMag Human Naive CD4+ T cell Enrichment Set - DM Cat. No. 558521 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. In panels A and B, cells were stained with a cocktail consisting of PE Anti-Human CD8 Cat. No. 555367 , CD11b Cat. No. 555388 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD36 Cat. No. 555455 , CD45RO Cat. No. 555493 , CD56 Cat. No. 555516 , CD123 Cat. No. 555644 , CD235a Cat. No. 555570 , and gammadelta TCR Cat No. 555717 to detect non-naive CD4+ cells, and an APC Human CD45RA Cat. No. 550855 to detect naive cells. For panels C and D, cells were stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349 and FITC Mouse Anti-Human CD45RA antibody Cat. No. 555488 to show the percentage of naive CD4+ T cells. Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Refer to the Enrichment Flow Chart to identify the cell populations in this figure. The percentage of naive CD4+ T cells is indicated in each panel. Panels A and C show unseparated PBMCs while panels B and D show the twice-enriched fraction after three 6-minute magnetic separations with an additional 3-10 minute separation. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Enrichment of naive CD4+ T cells from human peripheral blood. PBMC were labeled with the BD IMag Human Naive CD4+ T cell Enrichment Set - DM Cat. No. 558521 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. In panels A and B, cells were stained with a cocktail consisting of PE Anti-Human CD8 Cat. No. 555367 , CD11b Cat. No. 555388 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD36 Cat. No. 555455 , CD45RO Cat. No. 555493 , CD56 Cat. No. 555516 , CD123 Cat. No. 555644 , CD235a Cat. No. 555570 , and gammadelta TCR Cat No. 555717 to detect non-naive CD4+ cells, and an APC Human CD45RA Cat. No. 550855 to detect naive cells. For panels C and D, cells were stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349 and FITC Mouse Anti-Human CD45RA antibody Cat. No. 555488 to show the percentage of naive CD4+ T cells. Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Refer to the Enrichment Flow Chart to identify the cell populations in this figure. The percentage of naive CD4+ T cells is indicated in each panel. Panels A and C show unseparated PBMCs while panels B and D show the twice-enriched fraction after three 6-minute magnetic separations with an additional 3-10 minute separation. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.