BD IMagTM Human Lineage Cell Depletion Set - DM

Description

Depletion of lineage cells from human peripheral blood. PBMC were labeled with the BD IMag Human Lineage Cell Depletion Set - DM Cat. No. 560030 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a human lineage cocktail consisting of PE Mouse Anti-Human CD3 Cat. No. 555333 , CD14 Cat. No. 555398 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD56 Cat. No. 555516 , CD235a Cat. No. 555570 to detect lineage cells, and an APC Mouse Anti-Human CD45 Cat. No. 555483 to detect non-lineage cells. Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart on the next page to identify the cell populations represented in this figure. The percentage of non-lineage cells is indicated in each panel. Left panel shows the unseparated PBMC while the middle and right panels show the twice-enriched fraction after three 6-minute magnetic separations with an additional 10 minute separation middle and the positive fraction right . Depletion of lineage cells from human peripheral blood. PBMC were labeled with the BD IMag Human Lineage Cell Depletion Set - DM Cat. No. 560030 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a human lineage cocktail consisting of PE Mouse Anti-Human CD3 Cat. No. 555333 , CD14 Cat. No. 555398 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD56 Cat. No. 555516 , CD235a Cat. No. 555570 to detect lineage cells, and an APC Mouse Anti-Human CD45 Cat. No. 555483 to detect non-lineage cells. Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart on the next page to identify the cell populations represented in this figure. The percentage of non-lineage cells is indicated in each panel. Left panel shows the unseparated PBMC while the middle and right panels show the twice-enriched fraction after three 6-minute magnetic separations with an additional 10 minute separation middle and the positive fraction right . Show More Show More Depletion of lineage cells from human peripheral blood. PBMC were labeled with the BD IMag Human Lineage Cell Depletion Set - DM Cat. No. 560030 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a human lineage cocktail consisting of PE Mouse Anti-Human CD3 Cat. No. 555333 , CD14 Cat. No. 555398 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD56 Cat. No. 555516 , CD235a Cat. No. 555570 to detect lineage cells, and an APC Mouse Anti-Human CD45 Cat. No. 555483 to detect non-lineage cells. Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart on the next page to identify the cell populations represented in this figure. The percentage of non-lineage cells is indicated in each panel. Left panel shows the unseparated PBMC while the middle and right panels show the twice-enriched fraction after three 6-minute magnetic separations with an additional 10 minute separation middle and the positive fraction right .