BD IMagTM Human B Lymphocyte Enrichment Set - DM

Description

Enrichment of B lymphocytes from PBMC derived from two different donors. Leukocytes were labeled with the BD IMag Human B Lymphocyte Enrichment Set - DM Cat. No. 558007 and separated using the BD IMag Cell Separation Magnet Cat. No. 552311 . Cells were stained with APC Mouse Anti-Human CD19 Cat. no. 555415 to detect B lymphocytes and a mixture of FITC Mouse Anti- Human CD3 Cat. No. 555332 and CD43 Cat. No. 555475 to detect non-B leukocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No.556463 , Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The percentages of CD19+ CD3-CD43- B lymphocytes in each sample is given. The data from two separate donors top vs. bottom panels demonstrate that the additional separation step increases the purity of the enriched cells when the donor has a low frequency of B lymphocytes. Enrichment of B lymphocytes from PBMC derived from two different donors. Leukocytes were labeled with the BD IMag Human B Lymphocyte Enrichment Set - DM Cat. No. 558007 and separated using the BD IMag Cell Separation Magnet Cat. No. 552311 . Cells were stained with APC Mouse Anti-Human CD19 Cat. no. 555415 to detect B lymphocytes and a mixture of FITC Mouse Anti- Human CD3 Cat. No. 555332 and CD43 Cat. No. 555475 to detect non-B leukocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No.556463 , Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The percentages of CD19+ CD3-CD43- B lymphocytes in each sample is given. The data from two separate donors top vs. bottom panels demonstrate that the additional separation step increases the purity of the enriched cells when the donor has a low frequency of B lymphocytes. Show More Enrichment of B lymphocytes from PBMC derived from two different donors. Leukocytes were labeled with the BD IMag Human B Lymphocyte Enrichment Set - DM Cat. No. 558007 and separated using the BD IMag Cell Separation Magnet Cat. No. 552311 . Cells were stained with APC Mouse Anti-Human CD19 Cat. no. 555415 to detect B lymphocytes and a mixture of FITC Mouse Anti- Human CD3 Cat. No. 555332 and CD43 Cat. No. 555475 to detect non-B leukocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No.556463 , Flow cytometry was performed on a BD FACSCalibur flow cytometry system. The percentages of CD19+ CD3-CD43- B lymphocytes in each sample is given. The data from two separate donors top vs. bottom panels demonstrate that the additional separation step increases the purity of the enriched cells when the donor has a low frequency of B lymphocytes.