BD IMagTM Anti-Mouse CD90.2 Magnetic Particles - DM

Description

Positive selection and depletion of mouse CD90.2-positive splenocytes. Leukocytes were labeled with BD IMag anti-mouse CD90.2 Particles - DM Cat. No. 551518 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD90.2- and positive CD90.2+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh splenocytes left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC Anti-Mouse CD3e Cat. No. 553061 and PE Anti-mouse CD90.2 Cat. No. 553005 . The percent CD3e+/CD90.2+ cells in each sample is given in the upper right corner. Positive selection and depletion of mouse CD90.2-positive splenocytes. Leukocytes were labeled with BD IMag anti-mouse CD90.2 Particles - DM Cat. No. 551518 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD90.2- and positive CD90.2+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh splenocytes left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC Anti-Mouse CD3e Cat. No. 553061 and PE Anti-mouse CD90.2 Cat. No. 553005 . The percent CD3e+/CD90.2+ cells in each sample is given in the upper right corner. Show More Positive selection and depletion of mouse CD90.2-positive splenocytes. Leukocytes were labeled with BD IMag anti-mouse CD90.2 Particles - DM Cat. No. 551518 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD90.2- and positive CD90.2+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh splenocytes left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC Anti-Mouse CD3e Cat. No. 553061 and PE Anti-mouse CD90.2 Cat. No. 553005 . The percent CD3e+/CD90.2+ cells in each sample is given in the upper right corner.