BD HorizonTM V450 Mouse Anti-Human TNF

Description

Two-color flow cytometric analysis of TNF expression by activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours using Leukocyte Activation Cocktail, with BD GolgiPlug Cat. No. 550583 that contains Phorbol 12-Myristate 13-Acetate PMA , ionomycin and brefeldin A. The cells were then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with Alexa Fluor 700 Mouse Anti-Human CD3 Cat. No. 557943/561027 and either BD Horizon V450 Mouse Anti-Human TNF antibody Cat. No. 561311, Left Panel or BD Horizon V450 Mouse IgG1,kappa Isotype Control Cat. No. 560373, Right Panel . Two color flow cytometric dot plots showing the correlated expression of TNF or Ig Isotype Control Staining versus CD3 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. HiCK-1 Human Cytokine Positive Control cells Cat No. 555061 are prepared in a similar manner. These cells can be used as a positive control for cytokine flow cytometry experiments designed to characterize the nature of human TNF-producing cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of TNF expression by activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours using Leukocyte Activation Cocktail, with BD GolgiPlug Cat. No. 550583 that contains Phorbol 12-Myristate 13-Acetate PMA , ionomycin and brefeldin A. The cells were then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with Alexa Fluor 700 Mouse Anti-Human CD3 Cat. No. 557943/561027 and either BD Horizon V450 Mouse Anti-Human TNF antibody Cat. No. 561311, Left Panel or BD Horizon V450 Mouse IgG1,kappa Isotype Control Cat. No. 560373, Right Panel . Two color flow cytometric dot plots showing the correlated expression of TNF or Ig Isotype Control Staining versus CD3 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. HiCK-1 Human Cytokine Positive Control cells Cat No. 555061 are prepared in a similar manner. These cells can be used as a positive control for cytokine flow cytometry experiments designed to characterize the nature of human TNF-producing cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of TNF expression by activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours using Leukocyte Activation Cocktail, with BD GolgiPlug Cat. No. 550583 that contains Phorbol 12-Myristate 13-Acetate PMA , ionomycin and brefeldin A. The cells were then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with Alexa Fluor 700 Mouse Anti-Human CD3 Cat. No. 557943/561027 and either BD Horizon V450 Mouse Anti-Human TNF antibody Cat. No. 561311, Left Panel or BD Horizon V450 Mouse IgG1,kappa Isotype Control Cat. No. 560373, Right Panel . Two color flow cytometric dot plots showing the correlated expression of TNF or Ig Isotype Control Staining versus CD3 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. HiCK-1 Human Cytokine Positive Control cells Cat No. 555061 are prepared in a similar manner. These cells can be used as a positive control for cytokine flow cytometry experiments designed to characterize the nature of human TNF-producing cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of TNF expression by activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours using Leukocyte Activation Cocktail, with BD GolgiPlug Cat. No. 550583 that contains Phorbol 12-Myristate 13-Acetate PMA , ionomycin and brefeldin A. The cells were then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with Alexa Fluor 700 Mouse Anti-Human CD3 Cat. No. 557943/561027 and either BD Horizon V450 Mouse Anti-Human TNF antibody Cat. No. 561311, Left Panel or BD Horizon V450 Mouse IgG1,kappa Isotype Control Cat. No. 560373, Right Panel . Two color flow cytometric dot plots showing the correlated expression of TNF or Ig Isotype Control Staining versus CD3 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. HiCK-1 Human Cytokine Positive Control cells Cat No. 555061 are prepared in a similar manner. These cells can be used as a positive control for cytokine flow cytometry experiments designed to characterize the nature of human TNF-producing cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of TNF expression by activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours using Leukocyte Activation Cocktail, with BD GolgiPlug Cat. No. 550583 that contains Phorbol 12-Myristate 13-Acetate PMA , ionomycin and brefeldin A. The cells were then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with Alexa Fluor 700 Mouse Anti-Human CD3 Cat. No. 557943/561027 and either BD Horizon V450 Mouse Anti-Human TNF antibody Cat. No. 561311, Left Panel or BD Horizon V450 Mouse IgG1,kappa Isotype Control Cat. No. 560373, Right Panel . Two color flow cytometric dot plots showing the correlated expression of TNF or Ig Isotype Control Staining versus CD3 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. HiCK-1 Human Cytokine Positive Control cells Cat No. 555061 are prepared in a similar manner. These cells can be used as a positive control for cytokine flow cytometry experiments designed to characterize the nature of human TNF-producing cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of TNF expression by activated human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 4 hours using Leukocyte Activation Cocktail, with BD GolgiPlug Cat. No. 550583 that contains Phorbol 12-Myristate 13-Acetate PMA , ionomycin and brefeldin A. The cells were then fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained with Alexa Fluor 700 Mouse Anti-Human CD3 Cat. No. 557943/561027 and either BD Horizon V450 Mouse Anti-Human TNF antibody Cat. No. 561311, Left Panel or BD Horizon V450 Mouse IgG1,kappa Isotype Control Cat. No. 560373, Right Panel . Two color flow cytometric dot plots showing the correlated expression of TNF or Ig Isotype Control Staining versus CD3 were derived from events with the forward and side light-scatter characteristics of intact lymphocytes. HiCK-1 Human Cytokine Positive Control cells Cat No. 555061 are prepared in a similar manner. These cells can be used as a positive control for cytokine flow cytometry experiments designed to characterize the nature of human TNF-producing cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System.