BD HorizonTM RY775 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571663/571741; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 .The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571663/571741; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 .The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571663/571741; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 .The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571663/571741; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 .The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571663/571741; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 .The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571663/571741; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 .The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.