BD HorizonTM RY610 Mouse Anti-Human TIGIT

Description

Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 Cat. No.562752; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and Alexa Fluor 700 Mouse Anti-Human CD45RO Cat. No. 561136; Right Plots antibodies, and with either BD Horizon RY610 Mouse IgG1, kappa Isotype Control Cat. No. 571151; Top Plots or BD Horizon RY610 Mouse Anti-Human TIGIT antibody Cat. No. 571350/571354; Bottom Plots as indicated. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 was derived from gated events with the light-scatter characteristics of intact lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light-scatter characteristics of intact lymphocytes. Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 Cat. No.562752; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and Alexa Fluor 700 Mouse Anti-Human CD45RO Cat. No. 561136; Right Plots antibodies, and with either BD Horizon RY610 Mouse IgG1, kappa Isotype Control Cat. No. 571151; Top Plots or BD Horizon RY610 Mouse Anti-Human TIGIT antibody Cat. No. 571350/571354; Bottom Plots as indicated. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 was derived from gated events with the light-scatter characteristics of intact lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light-scatter characteristics of intact lymphocytes. Show More Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 Cat. No.562752; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and Alexa Fluor 700 Mouse Anti-Human CD45RO Cat. No. 561136; Right Plots antibodies, and with either BD Horizon RY610 Mouse IgG1, kappa Isotype Control Cat. No. 571151; Top Plots or BD Horizon RY610 Mouse Anti-Human TIGIT antibody Cat. No. 571350/571354; Bottom Plots as indicated. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 was derived from gated events with the light-scatter characteristics of intact lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light-scatter characteristics of intact lymphocytes.