BD HorizonTM RY610 Hamster Anti-Helios

Description

Two-color flow cytometric analysis of Helios expression Left Panel: Human peripheral blood mononuclear cells PBMCs were stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251; Left Plot or BD Horizon RY610 Hamster Anti-Helios antibody Cat. No. 571235/571299; Right Plot at 0.06 mug/test. Right Panel: Mouse splenic leukocytes were stained with BD Pharmingen APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set. The cells were then stained with either Hamster IgG1, kappa Isotype Control Left Plot or BD Horizon RY610 Hamster Anti-Helios antibody Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression of Helios or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact Human peripheral blood lymphocytes or splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Helios expression Left Panel: Human peripheral blood mononuclear cells PBMCs were stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251; Left Plot or BD Horizon RY610 Hamster Anti-Helios antibody Cat. No. 571235/571299; Right Plot at 0.06 mug/test. Right Panel: Mouse splenic leukocytes were stained with BD Pharmingen APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set. The cells were then stained with either Hamster IgG1, kappa Isotype Control Left Plot or BD Horizon RY610 Hamster Anti-Helios antibody Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression of Helios or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact Human peripheral blood lymphocytes or splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Helios expression Left Panel: Human peripheral blood mononuclear cells PBMCs were stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251; Left Plot or BD Horizon RY610 Hamster Anti-Helios antibody Cat. No. 571235/571299; Right Plot at 0.06 mug/test. Right Panel: Mouse splenic leukocytes were stained with BD Pharmingen APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set. The cells were then stained with either Hamster IgG1, kappa Isotype Control Left Plot or BD Horizon RY610 Hamster Anti-Helios antibody Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression of Helios or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact Human peripheral blood lymphocytes or splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.