BD HorizonTM RY586 Mouse Anti-Human CD184 (CXCR4)

Description

Multiparameter flow cytometric analysis of CD184 CXCR4 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human CD184 CXCR4 antibody Cat. No. 569646/569647; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD184 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo software. Multiparameter flow cytometric analysis of CD184 CXCR4 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human CD184 CXCR4 antibody Cat. No. 569646/569647; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD184 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo software. Multiparameter flow cytometric analysis of CD184 CXCR4 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human CD184 CXCR4 antibody Cat. No. 569646/569647; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD184 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo software. Show More Multiparameter flow cytometric analysis of CD184 CXCR4 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human CD184 CXCR4 antibody Cat. No. 569646/569647; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD184 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo software. Show More Multiparameter flow cytometric analysis of CD184 CXCR4 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human CD184 CXCR4 antibody Cat. No. 569646/569647; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD184 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo software. Multiparameter flow cytometric analysis of CD184 CXCR4 expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human CD184 CXCR4 antibody Cat. No. 569646/569647; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD184 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo software.